The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.
The Panda-Derived Lactobacillus plantarum G201683 Alleviates the Inflammatory Response in DSS-Induced Panda Microbiota-Associated Mice.
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DOI：
10.3389/fimmu.2021.747045
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发表时间：
2021
影响因子：
7.3
通讯作者：
Ni X
中科院分区：
文献类型：
--
作者：
Zhou Y;Duan L;Zeng Y;Niu L;Pu Y;Jacobs JP;Chang C;Wang J;Khalique A;Pan K;Fang J;Jing B;Zeng D;Ni X
肠道疾病是圈养大熊猫死亡的主要原因之一。其特殊的饮食习惯和胃肠道结构常导致肠道上皮损伤和继发性肠道感染。圈养大熊猫由于长期人工饲养和滥用抗生素，容易发生微生物群失调。然而，目前关于益生菌治疗大熊猫肠炎及相关的生态失调的报道较少。本研究旨在阐明植物乳杆菌（Lactobacillus plantarum）G201683（L. plantarum G83），一种有前途的熊猫来源的益生菌，在葡聚糖硫酸钠（DSS）诱导的熊猫微生物群相关（DPMA）小鼠模型中对肠道炎症发挥保护作用。采用抗生素治疗和5%DSS饮水的方法建立DPMA小鼠模型，观察L.植物G83对肠道炎症和肠道菌群的影响。我们的结果证明成功地建立了肠杆菌科富集的DPMA小鼠模型，与大熊猫肠道菌群一致。L. plantarum G83降低了DPMA小鼠结肠中肠道炎症的临床和组织学严重程度，增强了肠道紧密连接蛋白（ZO-1，Occludin）的表达，并减轻了炎性细胞因子（TNF-α）的产生。L.植物菌G83通过减少病原菌相关分类群如E.大肠杆菌和增加的丰富有益细菌包括双歧杆菌属。微生物群组成的这些变化与短链脂肪酸（SCFA）浓度增加、NF-κB信号传导减少和T辅助细胞亚群平衡改变有关。我们的研究结果支持L. plantarum G83是一种很有前途的治疗大熊猫肠道炎症的益生菌。
Intestinal diseases are one of the main causes of captive giant panda death. Their special dietary habits and gastrointestinal tract structure often lead to intestinal epithelium damage and secondary intestinal infection. The captive giant panda is predisposed to suffer from microbiota dysbiosis due to long-term artificial feeding and antibiotic misuse. However, there are few reported probiotics to treat giant panda enteritis and the associated dysbiosis. This study aims to elucidate the mechanism by which Lactobacillus plantarum G201683 (L. plantarum G83), a promising panda-derived probiotic, exerts a protective effect on intestinal inflammation in the dextran sulfate sodium- (DSS) induced panda microbiota-associated (DPMA) mouse model. The DPMA mouse was generated by antibiotic treatment and 5% DSS drinking water administration to assess the effect of L. plantarum G83 on intestinal inflammation and microbiota in vivo. Our results demonstrated the successful generation of a DPMA mouse model with Enterobacteriaceae enrichment, consistent with the giant panda intestinal microbiota. L. plantarum G83 decreased clinical and histological severity of intestinal inflammation, enhanced intestinal tight junction protein expression (ZO-1, Occludin) and alleviated inflammatory cytokine production (TNF-) in the colon of DPMA mice. The administration of L. plantarum G83 altered the microbiota composition by decreasing pathogen associated taxa such as E. coli and increasing abundance of beneficial bacteria including Bifidobacterium spp. These changes in microbiota composition were associated with an increased concentration of short chain fatty acids (SCFA), reduced NF-κB signaling, and an altered balance of T helper cell subsets. Our findings support L. plantarum G83 as a promising probiotic to treat intestinal inflammation in the giant panda.
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影响因子：
6.4
作者：
Cevallos SA;Lee JY;Velazquez EM;Foegeding NJ;Shelton CD;Tiffany CR;Parry BH;Stull-Lane AR;Olsan EE;Savage HP;Nguyen H;Ghanaat SS;Byndloss AJ;Agu IO;Tsolis RM;Byndloss MX;Bäumler AJ
通讯作者：
Bäumler AJ
影响因子：
100.3
作者：
Klaenhammer, Todd R.;Kleerebezem, Michiel;Rescigno, Maria
通讯作者：
Rescigno, Maria
影响因子：
2.8
作者：
Eun, Chang Soo;Kim, Yong Seok;Park, Yoon Kyung
通讯作者：
Park, Yoon Kyung
影响因子：
3.3
作者：
Eun, Su-Hyeon;Lim, Su-Min;Kim, Dong-Hyun
通讯作者：
Kim, Dong-Hyun
影响因子：
3.5
作者：
Guo, Wei;Mishra, Sudhanshu;Li, Ying
通讯作者：
Li, Ying