Protocol for the isolation and culture of mouse dorsal root ganglion neurons for imaging applications.
Protocol for the isolation and culture of mouse dorsal root ganglion neurons for imaging applications.
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DOI：
10.1016/j.xpro.2023.102717
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发表时间：
2023-12-15
期刊：
影响因子：
--
通讯作者：
Campbell, Zachary T.
中科院分区：
文献类型：
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作者：
Smith, Patrick R.;Meyer, Angela;Loerch, Sarah;Campbell, Zachary T.
感觉神经元在整个生物学中发挥着普遍的作用。探索其功能的体外研究取决于原代细胞培养的成功应用。在这里，我们提出了一个协议的分离和培养小鼠背根神经节神经元成像应用。我们描述了提取背根神经节，制备培养物，在体外维持数天，并进行免疫细胞化学标记的步骤。我们还包括额外下游应用方面的特殊考虑。有关本方案使用和执行的完整详细信息，请参见Smith等人。（2021年）。如何从小鼠中培养初级感觉神经元的详细方案初级培养物适合成像应用孵育最多6天允许神经突生长出版商注：进行任何实验方案都需要遵守当地机构的实验室安全和道德准则。感觉神经元在整个生物学中发挥着普遍的作用。探索其功能的体外研究取决于原代细胞培养的成功应用。在这里，我们提出了一个协议的分离和培养小鼠背根神经节神经元成像应用。我们描述了提取背根神经节，制备培养物，在体外维持数天，并进行免疫细胞化学标记的步骤。我们还包括额外下游应用方面的特殊考虑。
Sensory neurons play pervasive roles throughout biology. In vitro studies to probe their functions hinge on the successful application of primary cell culture. Here, we present a protocol for the isolation and culture of mouse dorsal root ganglion neurons for imaging applications. We describe steps for extracting dorsal root ganglia, preparing cultures, maintaining them for days in vitro, and performing immunocytochemical labeling. We also include special considerations with respect to additional downstream applications. For complete details on the use and execution of this protocol, please refer to Smith et al. (2021). Detailed protocol for how to culture primary sensory neurons from mice Primary cultures are amenable for imaging applications Incubation for up to six days allows for neurite outgrowth Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Sensory neurons play pervasive roles throughout biology. In vitro studies to probe their functions hinge on the successful application of primary cell culture. Here, we present a protocol for the isolation and culture of mouse dorsal root ganglion neurons for imaging applications. We describe steps for extracting dorsal root ganglia, preparing cultures, maintaining them for days in vitro, and performing immunocytochemical labeling. We also include special considerations with respect to additional downstream applications.
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DOI：
10.1007/978-1-4939-7237-1_15
发表时间：
2017
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
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作者：
Katzenell S;Cabrera JR;North BJ;Leib DA
通讯作者：
Leib DA
影响因子：
5.3
作者：
de la Pena, June Bryan;Chase, Rebecca;Campbell, Zachary T.
通讯作者：
Campbell, Zachary T.
影响因子：
5.3
作者：
de la Pena, June Bryan;Barragan-Iglesias, Paulino;Campbell, Zachary T.
通讯作者：
Campbell, Zachary T.
DOI：
10.1096/fj.202101933rr
发表时间：
2022-07
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
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作者：
通讯作者：
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影响因子：
7.3
作者：
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