Gene and noncoding RNA regulation underlying photoreceptor protection: microarray study of dietary antioxidant saffron and photobiomodulation in rat retina
Gene and noncoding RNA regulation underlying photoreceptor protection: microarray study of dietary antioxidant saffron and photobiomodulation in rat retina
复制标题
光感受器保护的基因和非编码RNA调控：饮食抗氧化剂藏红花和大鼠视网膜光生物调节的微阵列研究
作者：
R. Natoli;Yuan Zhu;K. Valter;S. Bisti;J. Eells;J. Stone
目的鉴定膳食抗氧化剂（Dopterron）和光生物调节（PBM）的神经保护作用相关基因和非编码RNA（ncRNA）。方法我们使用先前发表的光感受器损伤的测定，其中白化病Sprague道利大鼠在昏暗的周期性照明（12小时5勒克斯，12小时黑暗）中饲养，通过暴露于明亮（1,000勒克斯）的光24小时进行攻击。实验组通过用饮食中的阿司米龙（1 mg/kg/天，持续21天）或PBM（9 J/cm 2，在眼睛处，每天，持续5天）预处理来保护免受光损伤。将来自六个实验组（对照组、光损伤[LD]组、光损伤组、PBM组、光损伤组LD组和PBMLD组）中的每一组中的四只动物的一只眼睛的RNA与Affytron大鼠基因组ST阵列杂交。14个选择的基因的定量实时PCR分析用于验证微阵列结果。结果LD对175个基因和ncRNA的调控超过标准水平（与对照组相比p<0.05，倍数变化>2）。PBM预处理降低了这175个LD调节实体中126个的表达低于标准;西司他丁预处理降低了53个实体的表达（50个与PBM相同）。此外，PBM预处理调节了67个不受LD调节的实体的表达，而Escherichron预处理调节了122个不受LD调节的实体（48个与PBM相同）。在没有LD的情况下给予PBM和Pestron，调节基因和ncRNA超过标准水平，但数量少于其保护作用期间。高比例的LD调控的实体（>90%）是已知的基因。相比之下，ncRNA在PBM和Pastron调节的实体中的神经保护作用突出（分别为73%和62%）。结论：单独给药时，Pastron和PBM（更显著）都调节了大量的基因和ncRNA。在视网膜暴露于破坏性光之前给予，因此在发挥其神经保护作用的同时，它们调节了大量的实体，其中ncRNA是突出的。此外，已知基因和ncRNA的下调在两种神经保护剂的保护作用中是突出的。这些比较提供了两种神经保护剂诱导的基因表达的概述，并为更集中地研究其机制提供了基础。
Purpose To identify the genes and noncoding RNAs (ncRNAs) involved in the neuroprotective actions of a dietary antioxidant (saffron) and of photobiomodulation (PBM). Methods We used a previously published assay of photoreceptor damage, in which albino Sprague Dawley rats raised in dim cyclic illumination (12 h 5 lux, 12 h darkness) were challenged by 24 h exposure to bright (1,000 lux) light. Experimental groups were protected against light damage by pretreatment with dietary saffron (1 mg/kg/day for 21 days) or PBM (9 J/cm2 at the eye, daily for 5 days). RNA from one eye of four animals in each of the six experimental groups (control, light damage [LD], saffron, PBM, saffronLD, and PBMLD) was hybridized to Affymetrix rat genome ST arrays. Quantitative real-time PCR analysis of 14 selected genes was used to validate the microarray results. Results LD caused the regulation of 175 entities (genes and ncRNAs) beyond criterion levels (p<0.05 in comparison with controls, fold-change >2). PBM pretreatment reduced the expression of 126 of these 175 LD-regulated entities below criterion; saffron pretreatment reduced the expression of 53 entities (50 in common with PBM). In addition, PBM pretreatment regulated the expression of 67 entities not regulated by LD, while saffron pretreatment regulated 122 entities not regulated by LD (48 in common with PBM). PBM and saffron, given without LD, regulated genes and ncRNAs beyond criterion levels, but in lesser numbers than during their protective action. A high proportion of the entities regulated by LD (>90%) were known genes. By contrast, ncRNAs were prominent among the entities regulated by PBM and saffron in their neuroprotective roles (73% and 62%, respectively). Conclusions Given alone, saffron and (more prominently) PBM both regulated significant numbers of genes and ncRNAs. Given before retinal exposure to damaging light, thus while exerting their neuroprotective action, they regulated much larger numbers of entities, among which ncRNAs were prominent. Further, the downregulation of known genes and of ncRNAs was prominent in the protective actions of both neuroprotectants. These comparisons provide an overview of gene expression induced by two neuroprotectants and provide a basis for the more focused study of their mechanisms.
DOI：
10.1097/00006982-199616040-00030
发表时间：
1995-02
影响因子：
11.1
作者：
R. K. Kutty;G. Kutty;B. Wiggert;G. Chader;R. Darrow;D. Organisciak
通讯作者：
R. K. Kutty;G. Kutty;B. Wiggert;G. Chader;R. Darrow;D. Organisciak
二甲基硫脲对大鼠视网膜光损伤的保护作用。
影响因子：
4.4
作者：
Organisciak,DT;Darrow,RM;Jiang,YI;Marak,GE;Blanks,JC
通讯作者：
Blanks,JC