Time-Lapse In Situ 3D Imaging Analysis of Human Enamel Demineralisation Using X-ray Synchrotron Tomography.
Time-Lapse In Situ 3D Imaging Analysis of Human Enamel Demineralisation Using X-ray Synchrotron Tomography.
复制标题
使用X射线同步器断层扫描对人搪瓷去矿化的原位3D成像分析。
DOI：
10.3390/dj11050130
复制
发表时间：
2023-05-09
影响因子：
2.6
通讯作者：
中科院分区：
文献类型：
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作者：
龋齿是一种慢性疾病，通过酸溶解（在牙釉质、牙本质和牙骨质中）和蛋白水解降解（牙本质和牙骨质）引起牙齿组织结构的改变，并产生重要的护理成本。由于釉质的分级结构导致复杂的结构改变，因此需要可视化和描述釉质上的酸溶解过程。这个过程从釉质表面开始，并向深层发展，这就需要研究内部釉质结构。通常采用人工脱矿来模拟实验过程。在本研究中，使用原子力显微镜进行表面分析，以及使用同步辐射X射线断层扫描在酸暴露期间重复扫描以生成延时可视化序列的3D内部分析，研究了人牙釉质的脱矿作用。从投影和虚拟切片的二维分析和釉质质量的3D分析提供了在杆和杆间物质水平的组织变化的细节。除了结构修饰的可视化之外，还测定了溶解速率，这证明了这些技术的可行性和有用性。釉质脱矿的时间分析不限于溶解，并且可以应用于其他实验条件，用于分析处理过的釉质或釉质脱矿。
Caries is a chronic disease that causes the alteration of the structure of dental tissues by acid dissolution (in enamel, dentine and cementum) and proteolytic degradation (dentine and cementum) and generates an important cost of care. There is a need to visualise and characterise the acid dissolution process on enamel due to its hierarchical structure leading to complex structural modifications. The process starts at the enamel surface and progresses into depth, which necessitates the study of the internal enamel structure. Artificial demineralisation is usually employed to simulate the process experimentally. In the present study, the demineralisation of human enamel was studied using surface analysis carried out with atomic force microscopy as well as 3D internal analysis using synchrotron X-ray tomography during acid exposure with repeated scans to generate a time-lapse visualisation sequence. Two-dimensional analysis from projections and virtual slices and 3D analysis of the enamel mass provided details of tissue changes at the level of the rods and inter-rod substance. In addition to the visualisation of structural modifications, the rate of dissolution was determined, which demonstrated the feasibility and usefulness of these techniques. The temporal analysis of enamel demineralisation is not limited to dissolution and can be applied to other experimental conditions for the analysis of treated enamel or remineralisation.
影响因子：
10.7
作者：
Salvati E;Besnard C;Harper RA;Moxham T;Shelton RM;Landini G;Korsunsky AM
通讯作者：
Korsunsky AM