TRPA1s act as chemosensors but not as cold sensors or mechanosensors to trigger the swallowing reflex in rats.
TRPA1s act as chemosensors but not as cold sensors or mechanosensors to trigger the swallowing reflex in rats.
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DOI：
10.1038/s41598-022-07400-3
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发表时间：
2022-03-02
影响因子：
4.6
通讯作者：
Kitagawa J
中科院分区：
文献类型：
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作者：
Hossain MZ;Ando H;Unno S;Kitagawa J
我们研究了 TRPA1 在触发吞咽反射中的作用。 TRPA1 主要定位于吞咽相关区域的细神经纤维和成纤维细胞样细胞，以及结状-岩骨-颈静脉神经节复合体中的小至中型喉上神经传入神经。局部应用 TRPA1 激动剂异硫氰酸烯丙酯 (AITC)，剂量依赖性触发吞咽反射。先前局部应用 TRPA1 拮抗剂可显着减弱 AITC 诱导的反射。应用冷 AITC (4°C) 可以非常短暂地将现场温度降低至 < 17°C（TRPA1 可以被激活的温度），但对触发反射没有影响。相比之下，通过连续流动冷 AITC 或使用冰冻 AITC 将现场温度长时间降低至 1<17 °C，反而延迟/阻止了 AITC 诱发的反射的触发。先前应用 TRPA1 拮抗剂对点状机械刺激诱发的反射阈值或低力或高力连续机械压力刺激诱发的反射的数量没有影响。 TRPA1 具有功能性，可作为化学传感器，但不能作为冷传感器或机械传感器来触发吞咽反射。短暂的冷刺激对触发反射没有影响。然而，较长的冷刺激会因冷麻醉而延迟/阻止反射的触发。
We examined the role of TRPA1s in triggering the swallowing reflex. TRPA1s predominantly localized on thin nerve fibers and fibroblast-like cells in swallowing-related regions and on small to medium-sized superior laryngeal nerve-afferents in the nodose–petrosal–jugular ganglionic complex. Topical application of a TRPA1 agonist, allyl isothiocyanate (AITC), dose-dependently triggered swallowing reflexes. Prior topical application of a TRPA1 antagonist significantly attenuated the AITC-induced reflexes. Application of cold AITC (4 °C) very briefly reduced the on-site temperature to < 17 °C (temperature at which TRPA1s can be activated), but had no effect on triggering of the reflex. By contrast, reducing the on-site temperature to < 17 °C for a longer time by continuous flow of cold AITC or by application of iced AITC paradoxically delayed/prevented the triggering of AITC-induced reflexes. Prior application of the TRPA1 antagonist had no effect on the threshold for the punctate mechanical stimuli-induced reflex or the number of low-force or high-force continuous mechanical pressure stimuli-induced reflexes. TRPA1s are functional and act as chemosensors, but not as cold sensors or mechanosensors, for triggering of the swallowing reflex. A brief cold stimulus has no effect on triggering of the reflex. However, a longer cold stimulus delays/prevents triggering of the reflex because of cold anesthesia.
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影响因子：
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作者：
Dziewas R;Allescher HD;Aroyo I;Bartolome G;Beilenhoff U;Bohlender J;Breitbach-Snowdon H;Fheodoroff K;Glahn J;Heppner HJ;Hörmann K;Ledl C;Lücking C;Pokieser P;Schefold JC;Schröter-Morasch H;Schweikert K;Sparing R;Trapl-Grundschober M;Wallesch C;Warnecke T;Werner CJ;Weßling J;Wirth R;Pflug C
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DOI：
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发表时间：
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影响因子：
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