The Halophyte Halostachys caspica AP2/ERF Transcription Factor HcTOE3 Positively Regulates Freezing Tolerance in Arabidopsis.
The Halophyte Halostachys caspica AP2/ERF Transcription Factor HcTOE3 Positively Regulates Freezing Tolerance in Arabidopsis.
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盐生盐藻 AP2/ERF 转录因子 HcTOE3 正向调节拟南芥的耐冻性
DOI：
10.3389/fpls.2021.638788
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发表时间：
2021
影响因子：
5.6
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Yin F;Zeng Y;Ji J;Wang P;Zhang Y;Li W
aptala2 （AP2）和乙烯响应元件结合因子（ERF）基因家族是最大的植物特异性转录因子家族之一，在植物的发育进化和对各种胁迫的响应中起着至关重要的作用。TARGET OF EAT3 （TOE3）基因来源于海竹，属于AP2亚家族，具有两个AP2 dna结合结构域。目前，AP2家族主要在植物的生长和进化中起着至关重要的作用，但关于AP2在非生物胁迫耐受中的作用的报道很少。在这里，我们报道了一个新的冷调控转录因子基因HcTOE3，它对抗冻性有重要贡献。结果表明，高盐、干旱、极端温度（热、冷、冻）以及脱落酸和甲基紫胶等非生物胁迫均能强烈诱导海苔同化枝中HcTOE3的表达。过表达HcTOE3基因（OE）诱导转基因拟南芥耐冰冻胁迫。与野生型相比，OE系在冷冻处理下丙二醛含量和电解质泄漏较低，活性氧积累较少。然而，转基因植株的存活率、抗氧化酶活性和渗透调节物质脯氨酸含量均有所提高。此外，OE系通过上调冷响应基因（CBF1、CBF2、COR15、COR47、KIN1和RD29A）和脱落酸信号转导途径基因（ABI1、ABI2、ABI5和RAB18）的转录水平来提高抗冻能力。结果表明，HcTOE3正调控冰冻胁迫，作为提高植物抗冻性的候选基因具有很大的潜力。
The APETALA2 (AP2) and ethylene-responsive element-binding factor (ERF) gene family is one of the largest plant-specific transcription factor gene families, which plays a critical role in plant development and evolution, as well as response to various stresses. The TARGET OF EAT3 (TOE3) gene is derived from Halostachys caspica and belongs to the AP2 subfamily with two AP2 DNA-binding domains. Currently, AP2 family mainly plays crucial roles in plant growth and evolution, yet there are few reports about the role of AP2 in abiotic stress tolerance. Here, we report HcTOE3, a new cold-regulated transcription factor gene, which has an important contribution to freezing tolerance. The main results showed that the expression of HcTOE3 in the H. caspica assimilating branches was strongly induced by different abiotic stresses, including high salinity, drought, and extreme temperature (heat, chilling, and freezing), as well as abscisic acid and methyl viologen treatments. Overexpressing HcTOE3 gene (OE) induced transgenic Arabidopsis plant tolerance to freezing stress. Under freezing treatment, the OE lines showed lower content of malondialdehyde and electrolyte leakage and less accumulation of reactive oxygen species compared with the wild type. However, the survival rates, antioxidant enzyme activities, and contents of osmotic adjustment substance proline were enhanced in transgenic plants. Additionally, the OE lines increased freezing tolerance by up-regulating the transcription level of cold responsive genes (CBF1, CBF2, COR15, COR47, KIN1, and RD29A) and abscisic acid signal transduction pathway genes (ABI1, ABI2, ABI5, and RAB18). Our results suggested that HcTOE3 positively regulated freezing stress and has a great potential as a candidate gene to improve plant freezing tolerance.
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