Microwave Staining of Intestinal Whole-mount Preparations with Cuprolinic Blue
Microwave Staining of Intestinal Whole-mount Preparations with Cuprolinic Blue
复制标题
铜啉蓝微波染色肠整体制剂
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
A. Weyns
中科院分区：
文献类型：
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作者：
C. Van Ginneken;M. D. de Smet;F. Van Meir;A. Weyns
受微波对免疫组织化学反应具有有益影响的知识的鼓舞，本研究旨在了解微波是否可以改善肠道神经元的铜蛋白蓝染色以及已开发用于胃肠道整装制剂的实际方法。除了在Holst和Powley（1995）描述的方法中加入微波应用外，还进行了一些其他修改：在染色溶液中孵育前进行两次孵育和自由浮动孵育。在整装制备物中，大多数（如果不是全部）肠神经元被铜蛋白蓝染色。这些神经元表现为蓝绿色细胞，具有无反应的核和神经元突起。在高倍镜下，细胞质的特征是一个很好的蓝-绿色颗粒，细胞核中的核仁显示为一个蓝色的彩虹色结构。非特异性染色发生在纤维细胞和上皮细胞中，但由于它们的位置和外观，它们可以很容易地与神经元区分开来。修改的孵育和微波应用到传统的铜蛋白蓝染色方法的掺入变成一个易于使用的方法，似乎可视化大部分，如果不是全部，肠神经元在猪空肠的整装制剂。
Encouraged by the knowledge that microwaves have a beneficial effect on immunohistochemical reactions, the present study aimed to find out whether microwaves could improve the Cuprolinic Blue staining of enteric neurons as well as the actual method that has been developed for gastrointestinal whole-mount preparations. In addition to incorporating a microwave application in the method described by Holst and Powley (1995), some other modifications were made: two incubations before incubation in the staining solution and free-floating incubations. In the whole-mount preparations, most, if not all, enteric neurons were stained by Cuprolinic Blue. These neurons appeared as blue–green cells with non-reacting nuclei and neuronal processes. At higher magnification, the cytoplasm was characterized by a fine blue– green granulation, and the nucleolus in the nucleus appeared as a blue iridescent structure. Non-specific staining occurred in fibrocy tes and epithelial cells but, because of their location and appearance, they could easily be distinguished from neurons. The modified incubations and the incorporation of a microwave application into the conventional Cuprolinic Blue staining method turn the method into an easy-to-use one that seems to visualize most, if not all, enteric neurons in whole-mount preparations of the pig jejunum.
豚鼠回肠肠神经系统中的 c-Fos- 和 JunB- 免疫反应性。
影响因子：
1.7
作者：
Parr,EJ;Sharkey,KA
通讯作者：
Sharkey,KA