Metagenomic next-generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in HIV-infected Chinese adults.
Metagenomic next-generation sequencing versus traditional laboratory methods for the diagnosis of central nervous system opportunistic infections in HIV-infected Chinese adults.
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DOI：
10.1038/s41598-023-31974-1
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发表时间：
2023-03-25
影响因子：
4.6
通讯作者：
Xiong, Yong
中科院分区：
文献类型：
--
作者：
Deng, Liping;Li, Qian;Hu, Wenjia;Chen, Xiaoping;Chen, Tielong;Song, Shihui;Mo, Pingzheng;Zou, Shi;Zhang, Yongxi;Gao, Shicheng;Chen, Liangjun;Zhuang, Ke;Yang, Rongrong;Xiong, Yong
评估宏基因组下一代测序 (mNGS) 对患有中枢神经系统疾病的艾滋病毒/艾滋病 (PLWHA) 患者的临床价值。使用 mNGS 对 48 名患有 CNS 疾病的 PLWHA 的脑脊液 (CSF) 样本进行测序，并与临床常规诊断方法进行比较。总共有36/48 ss（75%）患者经mNGS诊断为病原体感染，且mNGS阳性检出比例高于临床常规诊断方法（75% vs 52.1%，X2 = 5.441，P = 0.020）。通过 mNGS，48 名患者中有 13 名 (27.1%) 检测出 3-7 种病原体。共检出病原菌77株，其中mNGS检出率为94.8%（73/77），临床常规方法检出率为37.0%（30/77）（X2 = 54.206，P < 0.001）。 mNGS检测病原菌的敏感性和特异性分别为63.9%（23/36）和66.7%（8/12），优于临床常规方法（23/36 vs 9/25，X2 = 4.601，P = 0.032；8/12 vs 5/23， X2 = 5.029，P = 0.009）。 mNGS的应用具有优越性，能够检测多种未知病原体和多种病原体感染，在诊断PLWHA中枢神经系统疾病方面具有较高的敏感性和特异性。
To evaluate clinical value of metagenomic next-generation sequencing (mNGS) in people living with HIV/AIDS (PLWHA) who had CNS disorders. Cerebrospinal fluid (CSF) samples from 48 PLWHA presenting with CNS disorders were sequenced using mNGS and compared with clinical conventional diagnostic methods. In total, 36/48 ss(75%) patients were diagnosed with pathogen(s) infection by mNGS, and the positive detection proportion by mNGS was higher than that by clinical conventional diagnostic methods (75% vs 52.1%, X2 = 5.441, P = 0.020). Thirteen out of 48 patients (27.1%) were detected with 3–7 pathogens by mNGS. Moreover, 77 pathogen strains were detected, of which 94.8% (73/77) by mNGS and 37.0% (30/77) by clinical conventional methods (X2 = 54.206, P < 0.001). The sensitivity and specificity of pathogens detection by mNGS were 63.9% (23/36) and 66.7% (8/12), respectively, which were superior to that by clinical conventional methods (23/36 vs 9/25, X2 = 4.601, P = 0.032; 8/12 vs 5/23, X2 = 5.029, P = 0.009). The application of mNGS was superior for its ability to detect a variety of unknown pathogens and multiple pathogens infection, and relatively higher sensitivity and specificity in diagnosis of CNS disorders in PLWHA.
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影响因子：
11.8
作者：
Siddiqi, Omar K.;Ghebremichael, Musie;Koralnik, Igor J.
通讯作者：
Koralnik, Igor J.
影响因子：
2.2
作者：
Manuel Bello-Lopez, Juan;Leon-Garcia, Gregorio;Ibanez-Cervantes, Gabriela
通讯作者：
Ibanez-Cervantes, Gabriela
DOI：
10.1146/annurev-pathmechdis-012418-012751
发表时间：
2019-01-24
期刊：
Annual review of pathology
影响因子：
--
作者：
Gu W;Miller S;Chiu CY
通讯作者：
Chiu CY
影响因子：
5.5
作者：
Ji, Yongjia;Wang, Zhenyan;Lu, Hongzhou
通讯作者：
Lu, Hongzhou
影响因子：
158.5
作者：
Wilson, M. R.;Sample, H. A.;Chiu, C. Y.
通讯作者：
Chiu, C. Y.