Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease.
Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease.
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血清 microRNA181a：与细胞内细胞因子水平相关，是急性移植物抗宿主病的潜在生物标志物。
DOI：
10.1016/j.cyto.2016.05.021
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发表时间：
2016-09
期刊：
影响因子：
3.8
通讯作者：
Kong Fansheng
中科院分区：
文献类型：
--
作者：
Xie Linna;Zhou Fang;Liu Ximin;Fang Yuan;Yu Zhe;Song Ningxia;Kong Fansheng
本研究的目的是探讨淋巴细胞相关血清miRNA与急性移植物抗宿主病（aGVHD）发病机制的临床相关性，并评估miRNA的预测和预后价值。连续纳入在济南军区总医院接受同种异体外周血干细胞移植（allo-PBSCT）的患者。 aGVHD患者经过临床诊断和分级，分为训练集和测试集。收集血样，分离总RNA，并进行RT-PCR检测miRNA表达（miR-181a-3p、miR-214-3p和miR-326）。采用流式细胞仪检测细胞内细胞因子水平，并检测miRNA对aGVHD的疾病特异性。共有120名患者入院。 aGVHD 患者的血清 miR-181a 水平显着升高，并且与 aGVHD 的严重程度相关，但 miR-214 和 miR-326 则不相关。 aGVHD患者中IL-2、IL-22、IL-17a等细胞因子水平与miR-181a水平呈正相关，而血清IL-13水平与miR-181a水平呈负相关。此外，在肾移植后出现急性排斥反应的患者或脓毒症患者中未检测到miR-181a水平升高。 MiR-181a水平敏感且特异地升高，尤其是在严重aGVHD患者中。 MiR-181a可能是aGVHD患者识别、诊断和预后的潜在生物标志物。
The aim of this study was to investigate the clinical relevance of lymphocyte-related serum miRNAs to the pathogenesis of acute graft-versus-host disease (aGVHD) and evaluate the predictive and prognosis value of miRNAs. Consecutive patients who received allogeneic peripheral blood stem cell transplantation (allo-PBSCT) in General Hospital of Jinan Military District were enrolled. aGVHD patients were diagnosed and graded clinically, and divided into the training set and the testing set. Blood samples were collected, total RNA was isolated, and RT-PCR was performed for miRNA expression (miR-181a-3p, miR-214-3p and miR-326). Intracellular cytokines levels were assayed by flow cytometry, and the disease specificity assay of miRNAs for aGVHD was detected. A total of 120 patients were admitted. Serum level of miR-181a in aGVHD patients was highly increased and associated with the severity of aGVHD, but not miR-214 and miR-326. Levels of cytokines including IL-2, IL-22, and IL-17a were positively correlated with miR-181a level, while serum IL-13 level was negatively correlated with miR-181a level in aGVHD patients. Moreover, increased miR-181a level was not detected in patients with acute rejection after kidney transplantation or sepsis patients. MiR-181a level was sensitively and specifically increased, especially in severe aGVHD patients. MiR-181a may be a potential biomarker for the identification, diagnosis, and prognosis of aGVHD patients.
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DOI：
10.1007/978-3-319-03218-4_36
发表时间：
2016
期刊：
--
影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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