Severe Acute Respiratory Syndrome Coronavirus 2 Total and Subgenomic RNA Viral Load in Hospitalized Patients.
Severe Acute Respiratory Syndrome Coronavirus 2 Total and Subgenomic RNA Viral Load in Hospitalized Patients.
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DOI：
10.1093/infdis/jiab215
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发表时间：
2021-10-28
期刊：
影响因子：
--
通讯作者：
Lauring AS
中科院分区：
文献类型：
--
作者：
Dimcheff DE;Valesano AL;Rumfelt KE;Fitzsimmons WJ;Blair C;Mirabelli C;Petrie JG;Martin ET;Bhambhani C;Tewari M;Lauring AS
先前的研究表明，严重急性呼吸综合征冠状病毒2（SARS-CoV-2）RNA可以在感染后数周内检测到。这一发现的意义尚不清楚，在大多数患者中，并不代表活动性感染。亚基因组RNA的检测已被提出代表生产性感染，并可能是一个有用的标志物，用于监测感染性。我们使用定量逆转录聚合酶链反应（RT-qPCR），以量化总的和亚基因组核衣壳（sgN）和包膜（sgE）的成绩单在185 SARS-CoV-2阳性鼻咽拭子样本收集入院，并与症状持续时间。我们发现，所有的成绩单以相同的速度下降，但是，sgE成为其他成绩单之前检测不到。症状至阴性测试的中位持续时间对于sgE为14天，对于sgN为25天。与总RNA相比，亚基因组中存在线性下降，表明亚基因组转录本拷贝数依赖于总转录本的拷贝数。总和sgN之间的平均差异为16倍，总和sgE之间的平均差异为137倍。这种关系在症状持续期间是恒定的，允许从总拷贝数预测亚基因组拷贝数。亚基因组RNA在确定感染性方面可能并不比总RNA的拷贝数阈值更有用。严重急性呼吸综合征冠状病毒2型亚基因组RNA已被提出作为COVID-19患者感染性的标志物。我们对185个样本的亚基因组RNA转录本的分析表明，它在确定感染性方面并不比总RNA拷贝数阈值更有用。
Previous studies demonstrated that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be detected for weeks after infection. The significance of this finding is unclear and, in most patients, does not represent active infection. Detection of subgenomic RNA has been proposed to represent productive infection and may be a useful marker for monitoring infectivity. We used quantitative reverse-transcription polymerase chain reaction (RT-qPCR) to quantify total and subgenomic nucleocapsid (sgN) and envelope (sgE) transcripts in 185 SARS-CoV-2–positive nasopharyngeal swab samples collected on hospital admission and to relate to symptom duration. We find that all transcripts decline at the same rate; however, sgE becomes undetectable before other transcripts. The median duration of symptoms to a negative test is 14 days for sgE and 25 days for sgN. There is a linear decline in subgenomic compared to total RNA, suggesting that subgenomic transcript copy number is dependent on copy number of total transcripts. The mean difference between total and sgN is 16-fold and the mean difference between total and sgE is 137-fold. This relationship is constant over duration of symptoms, allowing prediction of subgenomic copy number from total copy number. Subgenomic RNA may be no more useful in determining infectivity than a copy number threshold determined for total RNA. Severe acute respiratory syndrome coronavirus 2 subgenomic RNA has been proposed as a marker of infectivity in patients with COVID-19. Our analysis of subgenomic RNA transcripts in 185 samples suggests that it is no more useful in determining infectivity than a total RNA copy number threshold.
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影响因子：
17.1
作者：
Speranza E;Williamson BN;Feldmann F;Sturdevant GL;Pérez-Pérez L;Meade-White K;Smith BJ;Lovaglio J;Martens C;Munster VJ;Okumura A;Shaia C;Feldmann H;Best SM;de Wit E
通讯作者：
de Wit E
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影响因子：
11.8
作者：
Perera RAPM;Tso E;Tsang OTY;Tsang DNC;Fung K;Leung YWY;Chin AWH;Chu DKW;Cheng SMS;Poon LLM;Chuang VWM;Peiris M
通讯作者：
Peiris M
影响因子：
5.4
作者：
Dagotto, Gabriel;Mercado, Noe B.;Barouch, Dan H.
通讯作者：
Barouch, Dan H.
影响因子：
11.8
作者：
Bullard, Jared;Dust, Kerry;Poliquin, Guillaume
通讯作者：
Poliquin, Guillaume
影响因子：
16.6
作者：
Alexandersen S;Chamings A;Bhatta TR
通讯作者：
Bhatta TR