Promoter DNA hypermethylation and gene repression in undifferentiated Arabidopsis cells.
Promoter DNA hypermethylation and gene repression in undifferentiated Arabidopsis cells.
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DOI：
10.1371/journal.pone.0003306
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发表时间：
2008-10-01
期刊：
影响因子：
3.7
通讯作者：
Fraga MF
中科院分区：
文献类型：
--
作者：
Berdasco M;Alcázar R;García-Ortiz MV;Ballestar E;Fernández AF;Roldán-Arjona T;Tiburcio AF;Altabella T;Buisine N;Quesneville H;Baudry A;Lepiniec L;Alaminos M;Rodríguez R;Lloyd A;Colot V;Bender J;Canal MJ;Esteller M;Fraga MF
维持和获得植物的多能细胞状态是组织再生和营养繁殖的关键。基于组蛋白的表观遗传机制对于调节这种未分化状态很重要。在这里，我们报告了利用遗传学和药理学的实验方法来证明拟南芥细胞悬浮液和愈伤组织由于启动子DNA超甲基化而特异性地抑制某些基因。我们发现，MAPK12、GSTU10和BXL1基因的启动子在愈伤组织细胞中发生超甲基化，并且超甲基化也影响细胞悬浮液中的TTG1、GSTF5、SUVH8、Fimbrin和CCD7基因。未分化细胞中的启动子高甲基化与组蛋白低乙酰化有关，主要发生在CpG位点。因此，我们发现这个过程特异性地依赖于MET1和DRM2甲基转移酶，就像DNA甲基转移酶突变所证明的那样。我们的结果表明，启动子DNA甲基化可能是植物细胞建立和/或维持未分化状态的另一重要表观遗传机制。
Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.
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影响因子：
56.9
作者：
Jacobsen, SE;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
DOI：
10.1196/annals.1395.005
发表时间：
2007-01-01
期刊：
BIOGERONTOLOGY: MECHANISMS AND INTERVENTIONS
影响因子：
--
作者：
Fraga, Mario F.;Agrelo, Ruben;Esteller, Manel
通讯作者：
Esteller, Manel
影响因子：
2.7
作者：
Grafi, Gideon;Ben-Meir, Hagit;Zemach, Assaf
通讯作者：
Zemach, Assaf
将RNA定向DNA甲基化的两步募集到串联重复序列。
影响因子：
9.8
作者：
Chan, Simon W-L;Zhang, Xiaoyu;Bernatavichute, Yana V;Jacobsen, Steven E
通讯作者：
Jacobsen, Steven E
影响因子：
9.2
作者：
Jacobsen, SE;Sakai, H;Meyerowitz, EM
通讯作者：
Meyerowitz, EM