Fine mapping and cloning of MT1, a novel allele of D10

Fine mapping and cloning of MT1, a novel allele of D10
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D10 的新型等位基因 MT1 的精细定位和克隆

DOI:
10.1016/j.pnsc.2009.05.006
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发表时间:
2009-12
期刊:
Progress in Natural Science
影响因子:
--
通讯作者:
Zhu, Jinyan
Zhu, Jinyan
中科院分区:
其他
文献类型:
--
作者:
Li, Zhengyi;Liang, Guohua;Gu, Fei;Gu, Minghong;Zhang, Honggen;Tang, Shuzhu;Zhou, Yong;Zhu, Jinyan

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水稻分蘖是粮食产量的重要决定因素。为了研究分蘖机制,我们对经 EMS 处理的粳稻品种中华 11 中鉴定出的多分蘖突变体 (mt1) 进行了表征。该突变体表现出提前的分蘖发育并且与野生型植物相比矮化。遗传分析和精细基因图谱表明,mt1 突变体受隐性基因控制,该基因位于 1 号染色体 AP003376 上的 29 kb 窗口上。该区域中的一个假定基因编码类胡萝卜素裂解双加氧酶 8 (CCD8),与 D10 等位。通过引入野生型 MT1 来补充 mt1 突变表型,并且野生型水稻中 MT1 的敲低模仿了突变表型。实时PCR分析表明MT1基因在茎中高表达,在腋芽、圆锥花序、叶和根中低表达。此外,MT1的表达明显受到反馈调节。
Rice tillering is an important determinant for grain production. To investigate the mechanism of tillering, we characterized a multiple tillering mutant (mt1) identified from the japonica variety, Zhonghua 11, treated with EMS. This mutant exhibits advanced tillering development and dwarfed compared with wild-type plants. Genetic analysis and fine gene mapping indicated that the mt1 mutant was controlled by a recessive gene, residing on a 29-kb window on AP003376 of chromosome 1. One putative gene in this region, encoding a carotenoid cleavage dioxygenase 8 (CCD8), was allelic to D10. The mt1 mutant phenotype was complemented by introduction of wild-type MT1, and knockdown of MT1 in wild-type rice mimicked the mutant phenotype. Real-time PCR analysis indicated that the MT1 gene is expressed highly in stems and at a low level in axillary buds, panicles, leaves, and roots. In addition, MT1 expression is clearly under feedback regulation.
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