Sequential observation of mitochondrial distribution in mouse oocytes and embryos
Sequential observation of mitochondrial distribution in mouse oocytes and embryos
复制标题
小鼠卵母细胞和胚胎中线粒体分布的序贯观察
DOI：
10.1007/bf01228092
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发表时间：
1993
影响因子：
3.1
通讯作者：
T. Mori
中科院分区：
文献类型：
--
作者：
Takashi Tokura;Y. Noda;Y. Goto;T. Mori
本研究的目的是阐明通过细胞cycles.Materials和MethodsMouse小鼠卵母细胞和胚胎中的线粒体分布的变化，从小鼠和活体荧光线粒体染料罗丹明123染色。线粒体染色模式分为三种类型：结果在细胞周期中，卵母细胞和体内培养的胚胎的线粒体在细胞质中发生易位，在hCG前表现为H型，在hCG后8-9 h表现为PA型，在hCG后8-9 h表现为A型hCG后10-14小时再次出现H型，受精后hCG后24和31-32小时再次出现PA型。在双细胞期，hCG后35小时显示Ag模式，hCG后40小时观察到H模式，hCG后48小时观察到PA模式。在体外培养的胚胎，并显示发育阻滞，线粒体易位被抑制后，他们聚集在早期的两个细胞阶段（35小时后hCG）。此外，线粒体的易位恢复超氧化物歧化酶或硫氧还蛋白添加到培养基中。这两种酶已经被证明有能力克服developmentalblock.ConclusionThe本研究揭示，线粒体易位在细胞周期中，并建议，有一个线粒体易位和发育停滞之间的密切关系。
ObjectiveThe purpose of this study was to elucidate changes in the distribution of mitochondria through the cell cycle.Materials and MethodsMouse oocytes and embryos were recovered sequentially from mice and stained with the vital fluorescent mitochondrial stain rhodamine 123. Mitochondrial staining pattern were classified into three types: aggregation (Ag), homogeneous (H), and perinuclear accumulation (PA).ResultsSequential observations revealed that mitochondria of oocytes and embryos grown in vivo translocated in the cytoplasm during the cell cycle, showing the H pattern be fore human chorionic gonadotropin (hCG) administration, the PA pattern 8–9 hr post-hCG, the H pattern again 10–14 hr post-hCG, and the PA pattern again 24 and 31–32 hr post-hCG following fertilization. In the twocell stage, the Ag pattern was shown 35 hr post-hCG, the H pattern was observed 40 hr post-hCG, and the PA pattern was found 48 hr post-hCG. In the embryos cultured in vitro and showing developmental block, mitochondrial translocation was shown to be inhibited after they aggregated in the early two-cell stage (35 hr post-hCG). Moreover, the translocation of mitochondria was restored by the addition of superoxide dismutase or thioredoxin to the culture medium. Both of these enzymes have already been shown to have the ability to overcome developmental block.ConclusionThe present study revealed that mitochondria translocated in the cell cycle and suggested that there is a close relationship between mitochondrial translocation and developmental arrest.
DOI：
10.1073/pnas.82.12.4152
发表时间：
1985-01-01
影响因子：
11.1
作者：
SCHATTEN, G;SIMERLY, C;SCHATTEN, H
通讯作者：
SCHATTEN, H
影响因子：
2.7
作者：
PIKO, L;TAYLOR, KD
通讯作者：
TAYLOR, KD