Clonal drift of cell surface, melanogenic, and experimental metastatic properties of in vivo-selected, brain meninges-colonizing murine B16 melanoma.
Clonal drift of cell surface, melanogenic, and experimental metastatic properties of in vivo-selected, brain meninges-colonizing murine B16 melanoma.
复制标题
体内选择的脑膜定植鼠 B16 黑色素瘤的细胞表面克隆漂移、黑色素生成和实验转移特性。
作者：
K. M. Miner;T. Kawaguchi;G. W. Uba;G. Nicolson
摘要 使用肺定植潜力低的 B16 黑色素瘤细胞系 B16-F1，通过肿瘤细胞注射、收获脑表面肿瘤结节和体外组织培养生长的循环，选择亚系进行血源性植入、存活和在脑中生长。经过 14 次脑定植连续选择后，建立了亚系 B16-B14b，与 B16-F1 系相比，其形成脑肿瘤集落的能力增强。 B16-B14b 黑色素瘤脑肿瘤集落在静脉注射后形成。肿瘤细胞接种主要位于蛛网膜组织的脑动脉，普遍在血管内生长，侵入血管壁，在血管周围组织形成瘤灶。亚系 B16-B14b 在其大脑定植、生长和其他细胞特性方面相对稳定。然而，源自亚系 B16-B14b 的细胞克隆在细胞特性（黑色素含量、细胞形态和细胞表面蛋白）以及脑定植能力方面存在异质性。此外，细胞克隆在组织培养过程中的体外和体内特性的稳定性方面也存在异质性。三个不稳定的 B16-B14b 克隆的等量混合物在组织培养物中生长大约 30 代，与单独生长相似代数并在接种前混合的细胞克隆相比，发现细胞混合物的脑定植特性稳定。通过乳过氧化物酶催化的 125I 碘化和十二烷基硫酸钠：聚丙烯酰胺平板凝胶电泳放射自显影检查暴露的细胞表面蛋白，发现在具有高脑定植潜力的 B16 亚系和克隆上，分子量约为 90,000 的成分的暴露量增加。当在组织培养生长过程中检查不稳定的 B16-B14b 克隆是否存在 Mr 90,000 成分时，发现随着克隆失去其大脑定植能力，其暴露量减少。
Abstract Using the B16 melanoma cell line B16-F1 of low lung colonization potential, sublines were selected for blood-borne implantation, survival, and growth in the brain by cycles of tumor cell injection, harvesting of brain surface tumor nodules, and in vitro tissue culture growth. After 14 sequential selections for brain colonization, subline B16-B14b was established which showed enhanced abilities to form brain tumor colonies compared to line B16-F1. The B16-B14b melanoma brain tumor colonies formed after i.v. tumor cell inoculations were located mainly in the cerebral arteries of the arachnoid tissue and grew generally in the blood vessels, invaded the vessel walls, and formed tumor foci in perivascular tissue. The subline B16-B14b was relatively stable in its brain colonization, growth, and other cellular properties. However, cell clones derived from subline B16-B14b were heterogeneous in their cellular properties (melanin contents, cell morphologies, and cell surface proteins) and also in their brain colonization abilities. In addition, the cell clones were also heterogeneous with respect to the stabilities of these in vitro and in vivo properties during growth in tissue culture. An equal mixture of three unstable B16-B14b clones was allowed to grow in tissue culture for approximately 30 passages, and the cell mixture was found to be stabilized in its brain colonization properties compared to the cell clones grown individually for a similar number of passages and mixed just prior to inoculation. Examination of exposed cell surface proteins by lactoperoxidase-catalyzed 125I iodination and sodium dodecyl sulfate:polyacrylamide slab gel electrophoresis autoradiography revealed increased exposures of a component with an approximate molecular weight of 90,000 on B16 sublines and clones with high brain colonization potentials. When the unstable B16-B14b clones were examined during tissue culture growth for the presence of the Mr 90,000 component, it was found to decrease in exposure as the clones lost their brain colonization abilities.
影响因子：
11.2
作者：
B. Miller;F. Miller;J. Leith;G. Heppner
通讯作者：
B. Miller;F. Miller;J. Leith;G. Heppner
影响因子：
11.2
作者：
B. Miller;F. Miller;G. Heppner
通讯作者：
B. Miller;F. Miller;G. Heppner