The effects of agitation intensity with and without continuous sparging on the growth and antibody production of hybridoma cells
The effects of agitation intensity with and without continuous sparging on the growth and antibody production of hybridoma cells
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连续鼓泡和不鼓泡的搅拌强度对杂交瘤细胞生长和抗体产生的影响
DOI：
10.1016/0168-1656(89)90128-4
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发表时间：
1989
影响因子：
4.1
通讯作者：
A. Emery
中科院分区：
文献类型：
--
作者：
Steve Oh;A. Nienow;M. Al‐Rubeai;A. Emery
3个鼠杂交瘤的反应，以增加搅拌速度从100到450转/分的螺旋桨和Rushton涡轮机在表面曝气生物反应器的1.4升已被确定与pO 2被保持在高于最低水平的20%。已经测量了细胞生长和活力、抗体产生、葡萄糖消耗、乳酸盐产生和代谢活性，并且发现在该速度范围内不受影响。扫描电子显微镜（SEM）显示，即使在最高强度的搅拌下，也没有可检测到的细胞损伤。然而，一旦培养物连续用空气鼓泡，净细胞生长速率和最大细胞数显著下降，该下降随着搅拌器速度的增加而增加。为了概括这些发现，并与文献报道进行比较，这个速度范围的湍流参数的影响进行了讨论。还考虑了扩大规模的影响。
The response of 3 murine hybridomas to increasing speeds of agitation from 100 to 450 rpm with propellers and Rushton turbines in surface-aerated bioreactors of 1.4 l has been determined with pO2being kept above a minimum level of 20%. Cell growth and viability, antibody production, glucose consumption, lactate production and metabolic activity have been measured and found to be unaffected over this range of speeds. Scanning electron micrographs (SEM) showed no detectable cell damage even at the highest intensity of agitation. However, once the culture was continuously sparged with air the net cell growth rate and the maximum cell number fell markedly, that fall increasing with increasing agitator speed. To generalise these findings and compare them with literature reports, the implications of this speed range on the turbulence parameters have been discussed. The implications for scale-up are also considered.
DOI：
10.1115/1.3138276
发表时间：
1981-01-01
影响因子：
1.7
作者：
DEWEY, CF;BUSSOLARI, SR;DAVIES, PF
通讯作者：
DAVIES, PF