Gene expression changes in the septum: possible implications for microRNAs in sculpting the maternal brain.
Gene expression changes in the septum: possible implications for microRNAs in sculpting the maternal brain.
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DOI：
10.1371/journal.pone.0038602
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gammie SC
中科院分区：
文献类型：
--
作者：
Zhao C;Saul MC;Driessen T;Gammie SC
从非母体状态到母体状态的转变的特征在于支持照顾后代的各种CNS改变。隔膜（包括外侧和内侧部分）是一个大脑区域，以前与各种情感和动机过程有关，包括产妇护理。在这项研究中，我们使用微阵列（SEMLER算法），以检查产后小鼠的中隔基因表达的变化，并采用基因集富集分析（GSEA），以确定可能的调节基因表达的改变。用实时定量PCR验证用微阵列分析鉴定为差异调节的感兴趣的基因。我们发现，脂肪酸结合蛋白7（Fabp7）和甘丙肽（Gal）下调，而胰岛素样生长因子结合蛋白3（Igfbp3）上调产后小鼠相比，处女女性。先前发现这些基因在哺乳期间在其他大脑区域受到差异调节。我们还发现了一些新基因的表达改变，这些基因以前与母亲的行为无关，但可能在产后过程中发挥作用，包括谷氨酸-氨连接酶（Glul）和生长抑素受体1（Sstr 1）（两者都在产后上调）。参与代谢、细胞分化或增殖的基因也表现出表达改变。出乎意料的是，富集分析揭示了大量的microRNA、转录因子或保守的结合位点（177个，校正P值<0.05），它们与母体上调基因显著相关，而没有一个与下调基因相关。MicroRNA与胎盘和乳腺发育有关，但这是第一个表明它们也可能在塑造母体大脑中发挥关键作用的迹象。总之，这项研究提供了新的见解基因（沿着可能的机制，为他们的监管），参与隔膜介导的适应在产后期间。
The transition from the non-maternal to the maternal state is characterized by a variety of CNS alterations that support the care of offspring. The septum (including lateral and medial portions) is a brain region previously linked to various emotional and motivational processes, including maternal care. In this study, we used microarrays (PLIER algorithm) to examine gene expression changes in the septum of postpartum mice and employed gene set enrichment analysis (GSEA) to identify possible regulators of altered gene expression. Genes of interest identified as differentially regulated with microarray analysis were validated with quantitative real-time PCR. We found that fatty acid binding protein 7 (Fabp7) and galanin (Gal) were downregulated, whereas insulin-like growth factor binding protein 3 (Igfbp3) was upregulated in postpartum mice compared to virgin females. These genes were previously found to be differentially regulated in other brain regions during lactation. We also identified altered expression of novel genes not previously linked to maternal behavior, but that could play a role in postpartum processes, including glutamate-ammonia ligase (Glul) and somatostatin receptor 1 (Sstr1) (both upregulated in postpartum). Genes implicated in metabolism, cell differentiation, or proliferation also exhibited altered expression. Unexpectedly, enrichment analysis revealed a high number of microRNAs, transcription factors, or conserved binding sites (177 with corrected P-value <0.05) that were significantly linked to maternal upregulated genes, while none were linked to downregulated genes. MicroRNAs have been linked to placenta and mammary gland development, but this is the first indication they may also play a key role in sculpting the maternal brain. Together, this study provides new insights into genes (along with possible mechanisms for their regulation) that are involved in septum-mediated adaptations during the postpartum period.
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影响因子：
3.7
作者：
Gerstner JR;Vanderheyden WM;Shaw PJ;Landry CF;Yin JC
通讯作者：
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