Simultaneous Determination of Hemoglobin and Myoglobin Oxygen Binding Curves by Spectral Curve Fitting
Simultaneous Determination of Hemoglobin and Myoglobin Oxygen Binding Curves by Spectral Curve Fitting
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通过光谱曲线拟合同时测定血红蛋白和肌红蛋白氧结合曲线
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
J. Kelly
中科院分区：
文献类型：
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作者：
C. Barlow;K. Kelly;J. Kelly
A completely optical method is described for the simultaneous determination of hemoglobin and myoglobin oxygen saturation. Solution oxygen concentrations were computed from measurements of phosphorescence decay of a soluble palladium porphyrin using the Stern-Volmer quenching relationship. Visible absorption spectra were recorded of hemoglobin/myoglobin mixtures progressively deoxygenated by bacterial aerobic metabolism. Separate hemoglobin and myoglobin oxygenation curves were resolved from the spectra of solutions containing both proteins by curve fitting, with the use of singular value decomposition of the spectra vs. oxygen concentration matrix. The method yielded a P50 of 1.2 Torr for horse heart myoglobin at 24 ± 1°C, while the P50 for sheep hemoglobin was 23 Torr at the same temperature. These values, obtained from mixtures, compare favorably with literature values determined for hemoglobin and myoglobin separately.
通过一种新的测量氧浓度的光学方法测量线粒体氧化磷酸化的氧依赖性。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Wilson,DF;Rumsey,WL;Green,TJ;Vanderkooi,JM
通讯作者：
Vanderkooi,JM
影响因子：
56.9
作者：
RUMSEY, WL;VANDERKOOI, JM;WILSON, DF
通讯作者：
WILSON, DF
通过磷光成像观察离体灌注肝脏中的氧分布。
影响因子：
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作者：
Wilson,DF;Rumsey,WL;Vanderkooi,JM
通讯作者：
Vanderkooi,JM