Altering pyrroloquinoline quinone nutritional status modulates mitochondrial, lipid, and energy metabolism in rats.
Altering pyrroloquinoline quinone nutritional status modulates mitochondrial, lipid, and energy metabolism in rats.
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DOI：
10.1371/journal.pone.0021779
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Rucker RB
中科院分区：
文献类型：
--
作者：
Bauerly K;Harris C;Chowanadisai W;Graham J;Havel PJ;Tchaparian E;Satre M;Karliner JS;Rucker RB
我们已经报道了吡咯喹啉醌（PQQ）通过涉及线粒体相关细胞信号通路的机制改善动物的生殖、新生儿发育和线粒体功能。为了扩展这些观察，本文描述了PQQ对能量和脂质关系的影响以及对缺血再灌注损伤的明显保护。给Sprague-Dawley大鼠喂食营养全面的饲料，其中以0（PQQ-）或2 mg PQQ/Kg饲料（PQQ+）添加PQQ。测量包括：1）血清葡萄糖和胰岛素，2）每代谢体型的总能量消耗（Wt 3/4），3）呼吸抑制（在进食和禁食状态下），4）血浆脂质的变化，5）肝脏和心脏中的相对线粒体量，和6）与心脏缺血相关的指数。对于后者，大鼠（PQQ-或PQQ+）进行左前降支闭塞，然后再灌注2小时，以确定PQQ对梗死面积和心肌组织丙二醛水平的影响，丙二醛是脂质过氧化的指标。尽管未观察到血清葡萄糖、胰岛素和游离脂肪酸水平的显著差异，但PQQ−与PQQ+大鼠的能量消耗较低，且能量消耗（进食状态）与肝脏线粒体含量相关。在PQQ−大鼠与PQQ+大鼠中也观察到血浆二酰甘油和三酰甘油以及β-羟基丁酸浓度升高。此外，在2型糖尿病大鼠模型中，与生理盐水给药相比，PQQ给药（以4.5 mg/kg BW腹腔注射3天）导致6小时禁食期间血浆甘油三酯降低2倍以上。缺血/再灌注引起的心脏损伤在PQQ−大鼠中比在PQQ+大鼠中更明显。总的来说，这些数据表明，PQQ缺乏影响与正常线粒体功能相关的许多参数。
We have reported that pyrroloquinoline quinone (PQQ) improves reproduction, neonatal development, and mitochondrial function in animals by mechanisms that involve mitochondrial related cell signaling pathways. To extend these observations, the influence of PQQ on energy and lipid relationships and apparent protection against ischemia reperfusion injury are described herein. Sprague-Dawley rats were fed a nutritionally complete diet with PQQ added at either 0 (PQQ−) or 2 mg PQQ/Kg diet (PQQ+). Measurements included: 1) serum glucose and insulin, 2) total energy expenditure per metabolic body size (Wt3/4), 3) respiratory quotients (in the fed and fasted states), 4) changes in plasma lipids, 5) the relative mitochondrial amount in liver and heart, and 6) indices related to cardiac ischemia. For the latter, rats (PQQ− or PQQ+) were subjected to left anterior descending occlusions followed by 2 h of reperfusion to determine PQQ's influence on infarct size and myocardial tissue levels of malondialdehyde, an indicator of lipid peroxidation. Although no striking differences in serum glucose, insulin, and free fatty acid levels were observed, energy expenditure was lower in PQQ− vs. PQQ+ rats and energy expenditure (fed state) was correlated with the hepatic mitochondrial content. Elevations in plasma di- and triacylglyceride and β-hydroxybutryic acid concentrations were also observed in PQQ− rats vs. PQQ+ rats. Moreover, PQQ administration (i.p. at 4.5 mg/kg BW for 3 days) resulted in a greater than 2-fold decrease in plasma triglycerides during a 6-hour fast than saline administration in a rat model of type 2 diabetes. Cardiac injury resulting from ischemia/reperfusion was more pronounced in PQQ− rats than in PQQ+ rats. Collectively, these data demonstrate that PQQ deficiency impacts a number of parameters related to normal mitochondrial function.
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DOI：
10.1159/000457978
发表时间：
1965-01-01
期刊：
ENZYMOLOGIA BIOLOGICA ET CLINICA
影响因子：
--
作者：
BERGMEYER, HU;BERNT, E
通讯作者：
BERNT, E
影响因子：
4.2
作者：
Jump, DB;Botolin, D;Demeure, O
通讯作者：
Demeure, O
影响因子：
3.9
作者：
Chung, Sangwoon;Yao, Hongwei;Caito, Samuel;Hwang, Jae-Woong;Arunachalam, Gnanapragasam;Rahman, Irfan
通讯作者：
Rahman, Irfan
影响因子：
4.8
作者：
Erol, Erdal;Kumar, Leena S.;Binas, Bert
通讯作者：
Binas, Bert
影响因子：
2.1
作者：
Liu, SQ;Li, HH;Yang, JW
通讯作者：
Yang, JW