Potential diagnostic biomarkers for IgA nephropathy: a comparative study pre- and post-tonsillectomy
Potential diagnostic biomarkers for IgA nephropathy: a comparative study pre- and post-tonsillectomy
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IgA 肾病的潜在诊断生物标志物：扁桃体切除术前后的比较研究
DOI：
10.1007/s11255-016-1372-2
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发表时间：
2016-07
影响因子：
2
通讯作者：
Peng You-Ming
中科院分区：
文献类型：
--
作者：
Xie Ying-Xin;He Li-Yu;Chen Xian;Peng Xiao-Fei;Ye Mu-Yao;Zhao Yu-Jing;Yan Wen-Zhe;Liu Chan;Shao Jing;Peng You-Ming
目的：外周血单个核细胞（PBMCs）中BAFF、ST 6 GALNAC 2、C1 GALT 1和COSMC蛋白以及血浆IgA 1和半乳糖缺陷型IgA 1（Gd-IgA 1）水平是IgA肾病的潜在生物标志物。方法：收集16例IgAN患者扁桃体切除术前后外周血标本，采用免疫组化方法检测其血清中免疫球蛋白、IgA IgAN的诊断基于经皮肾活检组织的分析结果。外周血样本从三个没有肾脏疾病（非IgAN），扁桃体切除术前后，和16名健康对照进行了检查。采用实时荧光定量PCR检测PBMCs中BAFF、ST 6 GALNAC 2、C1 GALT 1和COSMC mRNA水平。ELISA法测定血浆IgA 1含量。结果：IgAN患者扁桃体切除术前BAFF、ST 6 GALNAC 2、C1 GALT 1、COSMC mRNA水平及血浆IgA 1、Gd-IgA 1浓度均显著高于健康对照组（P < 0.05）。扁桃体切除术后BAFF、ST 6 GALNAC 2的表达及血浆IgA 1水平显著升高，C1 GALT 1、COSMC的表达显著降低（P < 0.05）。然而，在非IgAN患者中，扁桃体切除术不影响BAFF、ST 6 GALNAC 2、C1 GALT 1和COSMC的mRNA水平，血浆IgA 1含量和Gd-IgA 1水平。结论：扁桃体切除术后，IgA肾病患者外周血单个核细胞中BAFF、ST 6 GALNAC 2、C1 GALT 1和COSMC的mRNA水平及血浆IgA 1水平均发生变化。BAFF和ST 6 GALNAC 2可能调节IgA 1的分泌和O-糖基化。
Objective:The proteins BAFF, ST6GALNAC2, C1GALT1, and COSMC in peripheral blood mononuclear cells (PBMCs) and plasma levels of IgA1 and galactose-deficient IgA1 (Gd-IgA1) are potential biomarkers for IgAN nephropathy. In this study, we comparatively studied the changes of those biomarkers before and after tonsillectomy.Methods:Peripheral blood samples were obtained from 16 IgAN patients with pre- and post-tonsillectomy. IgAN was diagnosed based on results from analysis of percutaneous renal biopsy tissue. Peripheral blood samples from three patients without renal diseases (non-IgAN), before and after tonsillectomy, and 16 healthy controls were also examined. BAFF, ST6GALNAC2, C1GALT1, and COSMC mRNA levels in PBMCs were detected using real-time PCR. Plasma IgA1 content was measured by ELISA. Gd-IgA1 levels were determined using the VV lectin-ELISA method.Results:BAFF, ST6GALNAC2, C1GALT1, and COSMC mRNA levels and the plasma concentrations of IgA1 and Gd-IgA1 in IgAN patients before tonsillectomy were significantly higher than those in healthy controls (P < 0.05). Tonsillectomy significantly increased the expression of BAFF and ST6GALNAC2, and plasma IgA1 level, while it downregulated that of C1GALT1 and COSMC (P < 0.05). However, in non-IgAN patients, tonsillectomy did not affect the mRNA levels of BAFF, ST6GALNAC2, C1GALT1, and COSMC, plasma IgA1 content and Gd-IgA1 level. Positive correlations were established between BAFF and IgA1 (r = 0.604, P < 0.01) and between ST6GALNAC2 and Gd-IgA1 (r = 0.623, P < 0.01).Conclusions:Tonsillectomy changes the mRNA levels of BAFF, ST6GALNAC2, C1GALT1, and COSMC in PBMCs, as well as the plasma IgA1 level in IgAN patients. BAFF and ST6GALNAC2 might regulate IgA1 secretion and O-glycosylation.
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DOI：
10.1093/oxfordjournals.qjmed.a068143
发表时间：
1987-09
期刊：
The Quarterly journal of medicine
影响因子：
--
作者：
Giuseppe D'Amico
通讯作者：
Giuseppe D'Amico
影响因子：
13.2
作者：
Liu, Lin-lin;Wang, Li-ning;Li, Xiao-li
通讯作者：
Li, Xiao-li
影响因子：
2.3
作者：
Kamei, Daigo;Moriyama, Takahito;Nitta, Kosaku
通讯作者：
Nitta, Kosaku
影响因子：
15.9
作者：
McCarthy, Douglas D.;Kujawa, Julie;Browning, Jeffrey L.
通讯作者：
Browning, Jeffrey L.
扁桃体切除术和类固醇治疗后，IgA肾病中肾肾血清半乳糖缺陷型IgA1的变化。
影响因子：
3.7
作者：
Nakata J;Suzuki Y;Suzuki H;Sato D;Kano T;Yanagawa H;Matsuzaki K;Horikoshi S;Novak J;Tomino Y
通讯作者：
Tomino Y