Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast
Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast
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鉴定一种新型核孔相关蛋白作为酵母中 HIV-1 Rev 蛋白的功能靶标
DOI：
10.1016/0092-8674(95)90438-7
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发表时间：
1995
期刊：
影响因子：
64.5
通讯作者：
M. Rosbash
中科院分区：
文献类型：
--
作者：
F. Stutz;M. Neville;M. Rosbash
HIV-1 Rev蛋白增加后生动物系统中未剪接和单剪接靶转录物的细胞质水平。基于实验表明Rev在酵母S.酿酒酵母，我们已经确定了酵母蛋白，与Rev的效应域相互作用的蛋白质，Riplp，是一种新的小核孔蛋白样蛋白，其中一些是与核孔。其最接近的已知酵母亲戚是核孔组件也牵连在mRNA从细胞核到细胞质的运输。过表达Riplp或缺失RIP1基因的菌株的分析表明，Rip1 p对于Rev对基因表达的影响是重要的，表明物理相互作用在体内具有功能意义。结果表明，Rev直接促进合适的转录本的细胞质运输，将它们靶向到核孔。
The HIV-1 Rev protein increases the cytoplasmic levels of unspliced and singly spliced target transcripts in metazoan systems. Based on experiments that indicate a similar function of Rev in the yeast S. cerevisiae, we have identified a yeast protein that interacts with the effector domain of Rev. The protein, Riplp, is a novel small nucleoporin-like protein, some of which is associated with nuclear pores. Its closest known yeast relative is a nuclear pore component also implicated in mRNA transport from nucleus to cytoplasm. Analysis of strains that overexpress Riplp or that are deleted for the RIP1 gene show that Rip1 p is important for the effect of Rev on gene expression, indicating that the physical interaction is of functional significance in vivo. The results suggest that Rev directly promotes the cytoplasmic transport of suitable transcripts by targeting them to the nuclear pore.
通过将细菌产生的蛋白质直接转移到人体细胞中来鉴定反式显性 HIV-1 rev 蛋白突变体。
影响因子：
14.9
作者：
Mermer,B;Felber,BK;Campbell,M;Pavlakis,GN
通讯作者：
Pavlakis,GN
DOI：
10.1073/pnas.86.5.1495
发表时间：
1989-03-01
影响因子：
11.1
作者：
FELBER, BK;HADZOPOULOUCLADARAS, M;PAVLAKIS, GN
通讯作者：
PAVLAKIS, GN
影响因子：
4.5
作者：
Lardelli, Rea M.;Thompson, James X.;Stevens, Scott W.
通讯作者：
Stevens, Scott W.