EMPTY PERICARP11 serves as a factor for splicing of mitochondrial nad1 intron and is required to ensure proper seed development in maize.
EMPTY PERICARP11 serves as a factor for splicing of mitochondrial nad1 intron and is required to ensure proper seed development in maize.
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EMPTY PERICARP11 是线粒体 nad1 内含子剪接的一个因子，是确保玉米种子正常发育所必需的
DOI：
10.1093/jxb/erx212
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发表时间：
2017-07-20
影响因子：
6.9
通讯作者：
Qiu F
中科院分区：
文献类型：
--
作者：
Ren X;Pan Z;Zhao H;Zhao J;Cai M;Li J;Zhang Z;Qiu F
玉米中的一个新的五肽重复序列（PPR）基因不同于以前报道的基因，因为它是nad1的所有四个内含子剪接所必需的。II组内含子在高等植物物种的线粒体基因组中是常见的。这些内含子的剪接是一个涉及多种因素协同作用的复杂过程。然而，这些因素很少在玉米中得到表征。在这项研究中，我们发现空果皮11（Emp11）基因，它编码的P型五肽重复（PPR）蛋白，是玉米种子发育所必需的。Emp 11功能的丧失严重损害胚和胚乳发育，导致玉米中的空果皮种子，并且Emp 11表达的改变导致籽粒大小和重量的数量变化。我们发现，emp11突变体表现出失败的nad1内含子剪接，表现出严重减少复合物I组装和活性，线粒体结构紊乱，并增加在交替氧化酶AOX2和AOX3水平。有趣的是，emp11表型在W22自交系中非常严重，但在B73 BC2F2分离穗中可以部分恢复。这些结果表明，EMP11作为一个因素的剪接线粒体nad1内含子，并需要线粒体功能，以确保适当的玉米种子发育。
A new pentatricopeptide repeat (PPR) gene in maize differs from previously reported genes, as it is required for splicing of all four introns of nad1. Group II introns are common in the mitochondrial genome of higher plant species. The splicing of these introns is a complex process involving the synergistic action of multiple factors. However, few of these factors have been characterized in maize. In this study, we found that the Empty pericarp11 (Emp11) gene, which encodes a P-type pentatricopeptide repeat (PPR) protein, is required for the development of maize seeds. The loss of Emp11 function seriously impairs embryo and endosperm development, resulting in empty pericarp seeds in maize, and alteration in Emp11 expression leads to quantitative variation in kernel size and weight. We found that the emp11 mutants showed a failure in nad1 intron splicing, exhibited a severe reduction in complex I assembly and activity, mitochondrial structure disturbances, and an increase in alternative oxidase AOX2 and AOX3 levels. Interestingly, the emp11 phenotype was very severe in the W22 inbred line but could be partially recovered in B73 BC2F2 segregating ears. These results suggest that EMP11 serves as a factor for the splicing of mitochondrial nad1 introns and is required for mitochondrial function to ensure proper seed development in maize.
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影响因子：
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