The sleeping beauty: how reproductive diapause affects hormone signaling, metabolism, immune response and somatic maintenance in Drosophila melanogaster.
The sleeping beauty: how reproductive diapause affects hormone signaling, metabolism, immune response and somatic maintenance in Drosophila melanogaster.
复制标题
DOI：
10.1371/journal.pone.0113051
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Nässel DR
中科院分区：
文献类型：
--
作者：
Kubrak OI;Kučerová L;Theopold U;Nässel DR
一些生物体可以通过进入休眠、滞育状态来适应季节性和其他环境挑战。因此，暴露在低温和短光周期下的昆虫会进入发育停滞、新陈代谢降低和抗逆性增强的状态。雌性黑腹果蝇在成年阶段可以进入浅度生殖滞育，这大大减少了机体的衰老，但人们对与这种休眠相关的生理学和内分泌学以及参与其调节的基因知之甚少。我们诱导黑腹果蝇滞育，并监测 12 周内对卵巢发育动态、碳水化合物和脂质代谢以及涉及内分泌信号、代谢和先天免疫的基因表达的影响。在滞育期间，食物摄入量急剧减少，但循环和储存的碳水化合物和脂质却升高。胰高血糖素和胰岛素样肽的基因转录物增加，并且这些肽的几个靶基因的表达也发生变化。先天免疫中的四个关键基因可以通过滞育果蝇的感染来诱导，其中两个，卓霉素和天蚕素 A1，在滞育过程中上调，与感染无关。滞育果蝇的死亡率非常低，寿命延长，肠上皮老化减少。滞育引起的许多表型在从滞育条件恢复一周后逆转。此外，缺乏特定胰岛素样肽（dilp5 和 dilp2-3）的突变果蝇表现出滞育发生率增加。我们的研究首次全面描述了黑腹果蝇生殖滞育的特征，并证明胰高血糖素和胰岛素样信号传导是休眠期间生理变化的关键调节因子之一。
Some organisms can adapt to seasonal and other environmental challenges by entering a state of dormancy, diapause. Thus, insects exposed to decreased temperature and short photoperiod enter a state of arrested development, lowered metabolism, and increased stress resistance. Drosophila melanogaster females can enter a shallow reproductive diapause in the adult stage, which drastically reduces organismal senescence, but little is known about the physiology and endocrinology associated with this dormancy, and the genes involved in its regulation. We induced diapause in D. melanogaster and monitored effects over 12 weeks on dynamics of ovary development, carbohydrate and lipid metabolism, as well as expression of genes involved in endocrine signaling, metabolism and innate immunity. During diapause food intake diminishes drastically, but circulating and stored carbohydrates and lipids are elevated. Gene transcripts of glucagon- and insulin-like peptides increase, and expression of several target genes of these peptides also change. Four key genes in innate immunity can be induced by infection in diapausing flies, and two of these, drosomycin and cecropin A1, are upregulated by diapause independently of infection. Diapausing flies display very low mortality, extended lifespan and decreased aging of the intestinal epithelium. Many phenotypes induced by diapause are reversed after one week of recovery from diapause conditions. Furthermore, mutant flies lacking specific insulin-like peptides (dilp5 and dilp2-3) display increased diapause incidence. Our study provides a first comprehensive characterization of reproductive diapause in D. melanogaster, and evidence that glucagon- and insulin-like signaling are among the key regulators of the altered physiology during this dormancy.
登录
查看更多内容
影响因子：
4.5
作者：
Biteau B;Karpac J;Supoyo S;Degennaro M;Lehmann R;Jasper H
通讯作者：
Jasper H
影响因子：
7.8
作者：
Bai H;Kang P;Tatar M
通讯作者：
Tatar M
影响因子：
3.9
作者：
Biteau, Benoit;Karpac, Jason;Hwangbo, DaeSung;Jasper, Heinrich
通讯作者：
Jasper, Heinrich
影响因子：
1.9
作者：
Anaka, Matthew;MacDonald, C. Danielle;Lloyd, Vett
通讯作者：
Lloyd, Vett
影响因子：
9.2
作者：
Brogiolo, W;Stocker, H;Hafen, E
通讯作者：
Hafen, E