Reducing l-lactate release from hippocampal astrocytes by intracellular oxidation increases novelty induced activity in mice.
Reducing l-lactate release from hippocampal astrocytes by intracellular oxidation increases novelty induced activity in mice.
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DOI：
10.1002/glia.23960
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发表时间：
2021-05
期刊：
影响因子：
6.2
通讯作者：
Teschemacher AG
中科院分区：
文献类型：
--
作者：
Vaccari Cardoso B;Shevelkin AV;Terrillion C;Mychko O;Mosienko V;Kasparov S;Pletnikov MV;Teschemacher AG
Astrocytes are in control of metabolic homeostasis in the brain and support and modulate neuronal function in various ways. Astrocyte‐derived l‐lactate (lactate) is thought to play a dual role as a metabolic and a signaling molecule in inter‐cellular communication. The biological significance of lactate release from astrocytes is poorly understood, largely because the tools to manipulate lactate levels in vivo are limited. We therefore developed new viral vectors for astrocyte‐specific expression of a mammalianized version of lactate oxidase (LOx) from Aerococcus viridans. LOx expression in astrocytes in vitro reduced their intracellular lactate levels as well as the release of lactate to the extracellular space. Selective expression of LOx in astrocytes of the dorsal hippocampus in mice resulted in increased locomotor activity in response to novel stimuli. Our findings suggest that a localized decreased intracellular lactate pool in hippocampal astrocytes could contribute to greater responsiveness to environmental novelty. We expect that use of this molecular tool to chronically limit astrocytic lactate release will significantly facilitate future studies into the roles and mechanisms of intercellular lactate communication in the brain. Lactate oxidase (LOx) expression limits the intra‐astrocytic pool of lactate and decreases its release. LOx in astrocytes of the dorsal mouse hippocampus causes increased activity in novel environment.
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DOI：
10.1126/science.1190721
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gourine AV;Kasymov V;Marina N;Tang F;Figueiredo MF;Lane S;Teschemacher AG;Spyer KM;Deisseroth K;Kasparov S
通讯作者：
Kasparov S
影响因子：
9.8
作者：
Lalo U;Palygin O;Rasooli-Nejad S;Andrew J;Haydon PG;Pankratov Y
通讯作者：
Pankratov Y
影响因子：
10.6
作者：
Abazyan, Bagrat;Nomura, Jun;Kannan, Geetha;Ishizuka, Koko;Tamashiro, Kellie L.;Nucifora, Frederick;Pogorelov, Vladimir;Ladenheim, Bruce;Yang, Chunxia;Krasnova, Irina N.;Cadet, Jean Lud;Pardo, Carlos;Mori, Susumu;Kamiya, Atsushi;Vogel, Michael W.;Sawa, Akira;Ross, Christopher A.;Pletnikov, Mikhail V.
通讯作者：
Pletnikov, Mikhail V.
影响因子：
29
作者：
Machler, Philipp;Wyss, Matthias T.;Weber, Bruno
通讯作者：
Weber, Bruno
DOI：
10.1161/hypertensionaha.114.04683
发表时间：
2015-04
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Marina N;Ang R;Machhada A;Kasymov V;Karagiannis A;Hosford PS;Mosienko V;Teschemacher AG;Vihko P;Paton JF;Kasparov S;Gourine AV
通讯作者：
Gourine AV