The Gulf Long-Term Follow-Up Study (GuLF STUDY): Biospecimen collection at enrollment.
The Gulf Long-Term Follow-Up Study (GuLF STUDY): Biospecimen collection at enrollment.
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DOI：
10.1080/15287394.2017.1283274
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Sandler DP
中科院分区：
文献类型：
--
作者：
Engel LS;Kwok RK;Miller AK;Blair A;Curry MD;McGrath JA;Sandler DP
2010年墨西哥湾深水地平线（DWH）爆炸导致有史以来最大的海上石油泄漏。GuLF研究正在调查与石油泄漏活动有关的可能的人类健康影响。该研究的目的之一是利用研究参与者的生物标本来调查与泄漏有关的健康影响。本文描述了在研究入组阶段采集、处理、运输和储存标本的方法。居住在海湾各州（亚拉巴马、佛罗里达、路易斯安那、密西西比和得克萨斯州东部）的GuLF研究参与者有资格在DWH爆炸后一到三年在登记时完成家访。在这次访问期间，收集了血液、尿液、脚趾甲和剪下的头发以及房屋灰尘样本。将标本置于装有低温和环境温度隔室的容器中过夜运输至中央处理实验室。然后将大多数血液和尿液样本等分并储存在液氮蒸气中或180 °C下，一些样本储存在120 °C下。共有11，193名参与者完成了家访，超过99%的参与者提供了至少一份生物标本。大多数参与者提供血液（93%），尿液（99%）和脚趾甲（89%），40%提供头发。几乎所有参与者（95%）都提供了房屋灰尘样本。大多数样本在采集后1天（58%）或2天（25%）由实验室接收。这些生物样本使得能够调查与泄漏相关的健康影响的一系列生物标志物，以及可能的与泄漏相关的暴露的一些生物标志物。生物标本采集、处理和储存方案旨在在后勤和预算限制范围内最大限度地提高当前和未来的科学价值，并可作为未来在类似的时间紧迫和地理分散环境中进行研究的模板。
The 2010 Deepwater Horizon (DWH) explosion in the Gulf of Mexico led to the largest ever marine oil spill by volume. The GuLF STUDY is investigating possible human health effects associated with oil spill activities. One objective of the study is to utilize biological specimens from study participants to investigate spill-related health effects. This paper describes the methods for collecting, processing, shipping, and storing specimens during the enrollment phase of the study. GuLF STUDY participants living in Gulf states (Alabama, Florida, Louisiana, Mississippi, and eastern Texas) were eligible to complete a home visit at enrollment, one to three years after the DWH explosion. During this visit, blood, urine, toenail and hair clippings, and house dust samples were collected. Specimens were shipped overnight to a central processing laboratory in containers with cold and ambient temperature compartments. Most blood and urine specimens were then aliquoted and stored in liquid nitrogen vapor or at ‒80°C, with some samples stored at ‒20°C. A total of 11,193 participants completed a home visit, and over 99% provided at least one biospecimen. Most participants provided blood (93%), urine (99%), and toenail clippings (89%), and 40% provided hair. Nearly all participants (95%) provided house dust samples. Most samples were received by the lab one (58%) or two (25%) days after collection. These biospecimens enable investigation of a range of biomarkers of spill-related health effects, and possibly some biomarkers of spill-related exposures. The biospecimen collection, handling, and storage protocols were designed to maximize current and future scientific value within logistical and budgetary constraints and can serve as a template for future studies conducted in similar time-critical and geographically dispersed settings.
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影响因子：
10.4
作者：
Kwok RK;Engel LS;Miller AK;Blair A;Curry MD;Jackson WB;Stewart PA;Stenzel MR;Birnbaum LS;Sandler DP;GuLF STUDY Research Team
通讯作者：
GuLF STUDY Research Team
影响因子：
11.4
作者：
Aurell, Johanna;Gullett, Brian K.
通讯作者：
Gullett, Brian K.
影响因子：
3.5
作者：
Costantini, Adele Seniori;Benvenuti, Alessandra;Miligi, Lucia
通讯作者：
Miligi, Lucia
影响因子：
4.5
作者：
Fenech, M
通讯作者：
Fenech, M
影响因子：
3.5
作者：
Fracasso, Maria Enrica;Doria, Denise;Manno, Maurizio
通讯作者：
Manno, Maurizio