Pulmonary exposure to single-walled carbon nanotubes does not affect the early immune response against Toxoplasma gondii.
Pulmonary exposure to single-walled carbon nanotubes does not affect the early immune response against Toxoplasma gondii.
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DOI：
10.1186/1743-8977-9-16
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发表时间：
2012-05-23
影响因子：
10
通讯作者：
Scheynius A
中科院分区：
文献类型：
--
作者：
Swedin L;Arrighi R;Andersson-Willman B;Murray A;Chen Y;Karlsson MC;Georén SK;Tkach AV;Shvedova AA;Fadeel B;Barragan A;Scheynius A
单壁碳纳米管（SWCNT）通过咽部抽吸或吸入给药后，在小鼠肺部引发明显的炎症和纤维化。人类在职业环境中暴露于单壁碳纳米管可能与感染同时发生，这可能会增强或抑制对违规代理的反应。在这里，我们研究了是否顺序暴露于SWCNT通过咽部抽吸和感染的小鼠与无处不在的细胞内寄生虫弓形虫会影响宿主对寄生虫的免疫反应。C57 BL/6小鼠通过连续两天咽部施用SWCNT（80 + 80 μg/小鼠）进行预暴露，随后静脉内注射1 × 103或1 × 104绿色荧光蛋白和表达荧光素酶的T.弓形虫速殖子T.通过真实的时间内的体内生物发光成像7天和通过空斑形成监测弓形虫。在支气管肺泡灌洗液（BAL）中分析炎症反应，并通过评估肺和脾的形态学变化和免疫反应来分析。不同感染小鼠体内寄生虫分布无明显差异。gondii或在寄生虫接种之前预先暴露于SWCNT 2天的那些小鼠。肺和脾组织学和BAL液中的炎症标志物反映了SWCNT暴露和T。弓形虫注射液。我们还注意到，CD 11 c阳性树突状细胞而非F4/80阳性巨噬细胞在咽部抽吸后9天在肺中保留SWCNT。然而，T.在肺和脾中未观察到CD 11 c和F4/80阳性细胞。预先暴露于SWCNT并不影响脾细胞对T.刚地。综上所述，我们的数据表明，预先暴露于SWCNT不会增强或抑制对T的早期免疫应答。小鼠弓形虫感染
Single-walled carbon nanotubes (SWCNT) trigger pronounced inflammation and fibrosis in the lungs of mice following administration via pharyngeal aspiration or inhalation. Human exposure to SWCNT in an occupational setting may occur in conjunction with infections and this could yield enhanced or suppressed responses to the offending agent. Here, we studied whether the sequential exposure to SWCNT via pharyngeal aspiration and infection of mice with the ubiquitous intracellular parasite Toxoplasma gondii would impact on the immune response of the host against the parasite. C57BL/6 mice were pre-exposed by pharyngeal administration of SWCNT (80 + 80 μg/mouse) for two consecutive days followed by intravenous injection with either 1x103 or 1x104 green fluorescence protein and luciferase-expressing T. gondii tachyzoites. The dissemination of T. gondii was monitored by in vivo bioluminescence imaging in real time for 7 days and by plaque formation. The inflammatory response was analysed in bronchoalveolar lavage (BAL) fluid, and by assessment of morphological changes and immune responses in lung and spleen. There were no differences in parasite distribution between mice only inoculated with T. gondii or those mice pre-exposed for 2 days to SWCNT before parasite inoculum. Lung and spleen histology and inflammation markers in BAL fluid reflected the effects of SWCNT exposure and T. gondii injection, respectively. We also noted that CD11c positive dendritic cells but not F4/80 positive macrophages retained SWCNT in the lungs 9 days after pharyngeal aspiration. However, co-localization of T. gondii with CD11c or F4/80 positive cells could not be observed in lungs or spleen. Pre-exposure to SWCNT did not affect the splenocyte response to T. gondii. Taken together, our data indicate that pre-exposure to SWCNT does not enhance or suppress the early immune response to T. gondii in mice.
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影响因子：
3.1
作者：
Saeij, JPJ;Boyle, JP;Boothroyd, JC
通讯作者：
Boothroyd, JC
影响因子：
4.5
作者：
Kim JO;Jung SS;Kim SY;Kim TY;Shin DW;Lee JH;Lee YH
通讯作者：
Lee YH
影响因子：
4.6
作者：
Fenoy, I.;Giovannoni, M.;Goldman, A.
通讯作者：
Goldman, A.
影响因子：
3.1
作者：
Channon, JY;Seguin, RM;Kasper, LH
通讯作者：
Kasper, LH
影响因子：
3.8
作者：
Mitchell, Leah A.;Gao, Jun;McDonald, Jacob D.
通讯作者：
McDonald, Jacob D.