5‐Hydroxytryptamine Induces TIS8/egr‐1 and c‐fos Expression in PC12 Cells. Involvement of Tyrosine Protein Phosphorylation
5‐Hydroxytryptamine Induces TIS8/egr‐1 and c‐fos Expression in PC12 Cells. Involvement of Tyrosine Protein Phosphorylation
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5-羟色胺诱导 PC12 细胞中 TIS8/egr-1 和 c-fos 表达参与酪氨酸蛋白磷酸化。
DOI：
10.1111/j.1460-9568.1997.tb01356.x
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发表时间：
1997
影响因子：
3.4
通讯作者：
J. Zwiller
中科院分区：
文献类型：
--
作者：
N. Humblot;L. Esteve;C. Burgun;D. Aunis;J. Zwiller
The TIS8/egr‐1 gene is a member of the class of immediate early genes. Originally discovered as a mitogen induced gene, it can also be induced by synaptic activity. We report here the induction of the TIS8/egr‐1 gene by the neurotransmitter 5‐hydroxytryptamine (5‐HT) in cultured rat phaeochromocytoma PC12 cells. Induction was maximal 40 min after addition of 5‐HT to the cells, and declined very rapidly to reach the basal level after 90 min. The electrophoretic mobility‐shift assay showed that induction of the TIS8/egr‐1 gene by 5‐HT was accompanied by increased Egr‐1 protein binding to its DNA consensus sequence. We found an overall correlation of 5‐HT‐induced egr‐1 expression with that of c‐fos expression. The kinetics of the ability of both gene products to bind to their respective DNA consensus sequence was also similar. The 5‐HT‐induced activation in Egr‐1 binding was inhibited by ketanserin and mesulergine, indicating that 5‐HT exerted its action via a 5‐HT2 receptor subtype. The tyrosine protein kinase inhibitor genistein abolished induction of the TIS8/egr‐1 gene, suggesting that tyrosine kinase activity is required for the induction of early genes by 5‐HT. Genistein also inhibited 5‐HT‐induced Egr‐1 binding activity. The increase in phosphotyrosine content of focal adhesion kinase we noticed upon addition of 5‐HT to cells suggests that this cytoplasmic tyrosine protein kinase mediates the effect of 5‐HT in eliciting early gene induction.
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影响因子：
8
作者：
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10.1073/pnas.89.15.6818
发表时间：
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影响因子：
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作者：
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DOI：
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1991-06
影响因子：
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影响因子：
8
作者：
Kujubu,DA;Lim,RW;Varnum,BC;Herschman,HR
通讯作者：
Herschman,HR
DOI：
10.1073/pnas.89.11.5192
发表时间：
1992-06-01
影响因子：
11.1
作者：
SCHALLER, MD;BORGMAN, CA;PARSONS, JT
通讯作者：
PARSONS, JT