Differential gene expression analysis and cytological evidence reveal a sexual stage of an amoeba with multiparental cellular and nuclear fusion.
Differential gene expression analysis and cytological evidence reveal a sexual stage of an amoeba with multiparental cellular and nuclear fusion.
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DOI：
10.1371/journal.pone.0235725
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Stewart AJ
中科院分区：
文献类型：
--
作者：
Tekle YI;Wang F;Heidari A;Stewart AJ
有性是真核生物的一个标志，但它在微生物真核生物中的进化很少被阐明。最近的基因组研究表明，微生物真核生物拥有有性繁殖所需的遗传工具箱。然而，包括阿米巴在内的大多数微生物真核生物的性发育机制还没有得到很好的描述。在微生物真核生物中研究性别的主要障碍是缺乏观察证据，这主要是由于其神秘的性质。在这项研究中，我们使用了一种易驯化的融合阿米巴，Cochliopodium，通过阶段特异性差异基因表达(DGE)和细胞学分析来研究性发育。DGE和细胞学结果都表明，在实验室培养中，大多数减数分裂和性别相关基因在融合的Cochliopodium中上调。在未融合和融合细胞中的相对基因本体论(GO)类别表示揭示了融合转录组向DNA代谢、核和连接酶的功能倾斜，这些都暗示着对性发育的承诺。然而，未融合细胞的GO类别由代谢途径和其他指示营养阶段的过程主导。我们的研究提供了强有力的证据，表明融合的细胞代表着Cochliopodium的有性阶段。我们的发现对理解其他具有相似生殖策略的真核生物中涉及多亲的遗传进化和机制具有进一步的意义。
Sex is a hallmark of eukaryotes but its evolution in microbial eukaryotes is poorly elucidated. Recent genomic studies revealed microbial eukaryotes possess a genetic toolkit necessary for sexual reproduction. However, the mechanism of sexual development in a majority of microbial eukaryotes including amoebozoans is poorly characterized. The major hurdle in studying sex in microbial eukaryotes is a lack of observational evidence, primarily due to its cryptic nature. In this study, we used a tractable fusing amoeba, Cochliopodium, to investigate sexual development using stage-specific Differential Gene Expression (DGE) and cytological analyses. Both DGE and cytological results showed that most of the meiosis and sex-related genes are upregulated in Cochliopodium undergoing fusion in laboratory culture. Relative gene ontology (GO) category representations in unfused and fused cells revealed a functional skew of the fused transcriptome toward DNA metabolism, nucleus and ligases that are suggestive of a commitment to sexual development. However, the GO categories of unfused cells were dominated by metabolic pathways and other processes indicative of a vegetative phase. Our study provides strong evidence that the fused cells represent a sexual stage in Cochliopodium. Our findings have further implications in understanding the evolution and mechanism of inheritance involving multiparents in other eukaryotes with a similar reproductive strategy.
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影响因子：
3.7
作者：
De Cádiz AE;Jeelani G;Nakada-Tsukui K;Caler E;Nozaki T
通讯作者：
Nozaki T
影响因子：
4
作者：
Ahmed, Emad A.;Sfeir, Agnel;Scherthan, Harry
通讯作者：
Scherthan, Harry
DOI：
10.1073/pnas.1814425116
发表时间：
2019-02-05
影响因子：
11.1
作者：
Bloomfield, Gareth;Paschke, Peggy;Urushihara, Hideko
通讯作者：
Urushihara, Hideko
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.