Otoprotective effects of erythropoietin on Cdh23erl/erl mice.
Otoprotective effects of erythropoietin on Cdh23erl/erl mice.
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DOI：
10.1016/j.neuroscience.2013.01.052
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发表时间：
2013-05-01
期刊：
影响因子：
3.3
通讯作者：
Zheng QY
中科院分区：
文献类型：
--
作者：
Han F;Yu H;Zheng T;Ma X;Zhao X;Li P;Le L;Su Y;Zheng QY
Cdh23erl/erl小鼠是一种新型的DFNB12小鼠模型，其特征是进行性听力损失。在本研究中，从出生后第7天开始，每隔一天对Cdh23erl/erl小鼠进行腹腔注射促红细胞生成素（EPO），持续7周。用磷酸盐缓冲液（PBS）处理或未处理的Cdh23erl/erl小鼠作为对照。在4周、6周和8周龄时对小鼠组测量听性脑干反应（ABR）阈值和畸变产物耳声发射（DPOAE）。结果表明，在三个时间点，在短声、8kHz、16kHz和32kHz刺激频率下，与未处理的小鼠相比，EPO能显著降低Cdh23erl/erl小鼠的ABR阈值。同时，在三个时间点，在15383Hz的f2频率下，EPO处理的Cdh23erl/erl小鼠组的DPOAE幅值显著高于未处理组。此外，在耳蜗中底回，EPO处理的Cdh23erl/erl小鼠的外毛细胞损失平均百分比显著低于未处理的小鼠（P < 0.05）。这是首次报道EPO在具有进行性听力损失的DFNB12小鼠模型中作为一种耳保护剂起作用。
The Cdh23erl/erl mice are a novel mouse model for DFNB12 and characterized by progressive hearing loss. In this study, erythropoietin (EPO) was given to the Cdh23erl/erl mice by intraperitoneally injection every other day from P7 for seven weeks. PBS treated or untreated Cdh23erl/erl mice were used as controls. Auditory-evoked brainstem response (ABR) thresholds and distortion product oto-acoustic emission (DPOAE) were measured in the mouse groups at age of 4, 6 and 8 weeks. The results show that EPO can significantly decrease the ABR thresholds in the Cdh23erl/erl mice as compared with those of the untreated mice at stimulus frequencies of click, 8-, 16- and 32- kHz at three time points. Meanwhile, DPOAE amplitudes in the EPO treated Cdh23erl/erl mouse group were significantly higher than those of the untreated groups at f2 frequency of 15,383 Hz at the three time points. Furthermore, the mean percentage of OHC loss at middle through basal turns of cochleae was significantly lower in EPO-treated Cdh23erl/erl mice than in the untreated mice (P < 0.05). This is the first report that EPO acts as an otoprotectant in a DFNB12 mouse model with progressive hearing loss.
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影响因子：
2.8
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.0900691106
发表时间：
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DOI：
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发表时间：
2001-03-27
影响因子：
11.1
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作者：
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