Decrease in hypothalamic Kiss1 and Kiss1r expression: a potential mechanism for fasting-induced suppression of the HPG axis in the adult male rhesus monkey (Macaca mulatta).
Decrease in hypothalamic Kiss1 and Kiss1r expression: a potential mechanism for fasting-induced suppression of the HPG axis in the adult male rhesus monkey (Macaca mulatta).
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DOI：
10.1055/s-0030-1269852
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发表时间：
2011-02
期刊：
影响因子：
--
通讯作者：
Shahab M
中科院分区：
文献类型：
--
作者：
Wahab F;Ullah F;Chan YM;Seminara SB;Shahab M
禁食通过不完全了解的机制抑制下丘脑-垂体-性腺（HPG）轴的功能。 2003年，下丘脑kisspeptin-Kiss1r信号被发现在调节HPG轴中发挥重要作用。我们最近发现，在成年雄性猕猴中，短期禁食会减弱 HPG 轴对外源 Kisspeptin 挑战的反应。在本研究中，我们通过检查成年雄性猕猴短期禁食和正常喂养条件下下丘脑表达的调节，探索了这种减弱反应的机制。从正常喂养的猴子 (n=3) 和禁食 48 小时的猴子 (n=3) 中提取下丘脑 mRNA。 Kiss1、Kiss1r 和 GnRH1 mRNA 通过逆转录和实时聚合酶链反应进行定量。此外，还收集血液样本以测量葡萄糖、皮质醇、瘦素和睾酮的血浆浓度。与进食动物相比，禁食动物的血浆葡萄糖、瘦素和睾酮水平降低，皮质醇水平升高。与进食猴子相比，禁食猴子的下丘脑 Kiss1 和 Kiss1r mRNA 表达显着降低（P<0.05），而两组之间下丘脑 GnRH1 mRNA 表达相当。因此，我们的结果表明，猴子短期禁食后下丘脑 Kiss1 和 Kiss1r 的表达减少。这种减少可能有助于灵长类动物禁食期间 HPG 轴的抑制。此外，我们在禁食猴子中发现 Kiss1r 表达较低，这为短期禁食期间 HPG 轴对外周 Kisspeptin 激发的反应减弱提供了解释。
Fasting suppresses functioning of the hypothalamic-pituitary-gonadal (HPG) axis by mechanisms that are incompletely understood. In 2003, hypothalamic kisspeptin-Kiss1r signaling was discovered to play a significant role in regulating the HPG axis. We have recently shown that in adult male macaques, short-term fasting attenuates the response of the HPG axis to an exogenous kisspeptin challenge. In the present study, we explored the mechanism underlying this attenuated response by examining the modulation of the hypothalamic expression of Kiss1 and Kiss1r under short-term fasting and normal feeding conditions in the adult male macaques. Hypothalamic mRNA was extracted from normal fed (n=3) and 48-h fasted (n=3) monkeys. Kiss1, Kiss1r, and GnRH1 mRNA was quantified by reverse transcription followed by real-time polymerase chain reaction. In addition, blood samples were collected for measurement of plasma concentrations of glucose, cortisol, leptin, and testosterone. In contrast to fed animals, plasma glucose, leptin, and testosterone levels decreased and cortisol levels increased in fasted animals. The hypothalamic expression of Kiss1 and Kiss1r mRNA was significantly lower (P<0.05) in fasted monkeys compared to fed monkeys while hypothalamic GnRH1 mRNA expression was comparable between the two groups. Thus, our results demonstrate that expression of hypothalamic Kiss1 and Kiss1r decrease after a short-term fasting in monkeys. This decrease may contribute to the suppression of the HPG axis during fasting conditions in primates. In addition, our finding of lower expression of Kiss1r in fasted monkeys provides an explanation for the attenuation in the HPG axis response to peripheral kisspeptin challenge during short-term fasting.
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DOI：
10.1073/pnas.1834399100
发表时间：
2003-09-16
影响因子：
11.1
作者：
de Roux, N;Genin, E;Milgrom, E
通讯作者：
Milgrom, E
影响因子：
4.8
作者：
Muir, AI;Chamberlain, L;Harrison, DC
通讯作者：
Harrison, DC
影响因子：
8.2
作者：
Hiden, Ursula;Bilban, Martin;Desoye, Gernot
通讯作者：
Desoye, Gernot
从 KISS1 到 Kisspeptins：历史视角和建议命名法。
影响因子：
3
作者：
Gottsch, Michelle L.;Clifton, Donald K.;Steiner, Robert A.
通讯作者：
Steiner, Robert A.
影响因子：
4.8
作者：
Gottsch, ML;Cunningham, MJ;Steiner, RA
通讯作者：
Steiner, RA