Validity testing of commercial urine cocaine metabolite assays: I. Assay detection times, individual excretion patterns, and kinetics after cocaine administration to humans.
Validity testing of commercial urine cocaine metabolite assays: I. Assay detection times, individual excretion patterns, and kinetics after cocaine administration to humans.
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商业尿液可卡因代谢物测定的有效性测试：I. 测定检测时间、个体排泄模式以及人类施用可卡因后的动力学。
DOI：
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发表时间：
1989
影响因子：
1.6
通讯作者：
J. Mitchell
中科院分区：
文献类型：
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作者：
E. Cone;S. Menchen;B. D. Paul;L. D. Mell;J. Mitchell
对从接受20 mg盐酸可卡因单次静脉给药的人类受试者中采集的临床标本进行了8种用于检测可卡因代谢物的商业尿液检测方法的有效性研究。在受控条件下采集标本，并在盲态条件下随机分析。还通过气相色谱/质谱法（GC/MS）测定每个样本中的苯甲酰芽子碱浓度。可卡因给药后最后一份阳性标本（大于或等于300 ng/mL苯甲酰芽子碱当量）的平均检测时间在7种商业检测中从16.9 h变化至52.9 h，按以下升序排列：Toxi-Lab小于TDx = EMIT dau = EMIT st小于Abuscreen小于Coat-A-Count =双抗体。相比之下，评价用于检测可卡因代谢物的商业斑点试验（KDI Quik试验）产生苯甲酰芽子碱的假阳性和假阴性，并且不被认为是检测可卡因代谢物的有效试验。四名受试者中苯甲酰爱子宁的排泄半衰期为5.9至7.9小时，苯甲酰爱子宁的总体回收率为可卡因给药剂量的15.0至34.3%。
A validity study of eight commercial urine assays for detection of cocaine metabolite was performed on clinical specimens collected from human subjects who received single 20-mg intravenous doses of cocaine hydrochloride. The specimens were collected under controlled conditions and analyzed in random order under blind conditions. Benzoylecgonine concentration in each specimen also was determined by gas chromatography/mass spectrometry (GC/MS). Mean times of detection of the last positive specimen (greater than or equal to 300 ng/mL of benzoylecgonine equivalents) after cocaine administration varied among seven of the commercial tests from 16.9 to 52.9 h in the following ascending order: Toxi-Lab less than TDx = EMIT dau = EMIT st less than Abuscreen less than Coat-A-Count = Double Antibody. In contrast, a commercial spot test (KDI Quik Test) which was evaluated for detection of cocaine metabolite produced both false positives and false negatives for benzoylecgonine and was not considered to be a valid test for detection of cocaine metabolite. Half-lives of excretion of benzoylecgonine among four subjects varied from 5.9 to 7.9 h, and overall recovery of benzoylecgonine varied from 15.0 to 34.3% of the administered dose of cocaine.
影响因子：
2.5
作者：
J. Ambre;M. Fischman;T. Ruo
通讯作者：
J. Ambre;M. Fischman;T. Ruo