Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/anillin.
Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/anillin.
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DOI：
10.1016/j.cub.2011.02.003
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发表时间：
2011-03-22
期刊：
影响因子：
9.2
通讯作者：
Paoletti, Anne
中科院分区：
文献类型：
--
作者：
Almonacid, Maria;Celton-Morizur, Severine;Jakubowski, Jennifer L.;Dingli, Florent;Loew, Damarys;Mayeux, Adeline;Chen, Jun-Song;Gould, Kathleen L.;Clifford, Dawn M.;Paoletti, Anne
在真核生物中，胞质分裂通常涉及肌动球蛋白环，其收缩促进子细胞分离。收缩环的组装在空间和时间上受到严格控制（参见综述）。在分裂酵母中，收缩环组件首先由苯胺样蛋白Mid1和Cdr2激酶组织成内侧皮质节点。这些节点然后横向合并成一个功能紧凑的收缩环。虽然Mid1在整个G2期存在于内侧皮质，但收缩环组件向节点的募集仅在有丝分裂开始时开始，这表明该事件是细胞周期调节的。波罗激酶是有丝分裂和胞质分裂的关键时间协调者，Polo样激酶Plo 1长期以来与Mid1调控有关：Plo 1在有丝分裂开始时激活Mid1核输出，将分裂平面特化与核位置偶联。在这里，我们提供的证据表明，Plo1也触发招聘的收缩环组件到内侧皮质节点。Plo1结合Mid1上至少两个独立的位点，包括Cdc2磷酸化的共有位点。Plo 1磷酸化Mid1前100个氨基酸内的几个残基，这些残基直接与IQGAP Rng 2相互作用，并影响肌球蛋白II募集的时间。Plo 1从而促进有丝分裂开始时的收缩环组装。
In eukaryotes, cytokinesis generally involves an acto-myosin ring, the contraction of which promotes daughter cells segregation. Assembly of the contractile ring is tightly controlled in space and time (see for reviews). In the fission yeast, contractile ring components are first organized by the Anillin-like protein Mid1 and Cdr2 kinase into medial cortical nodes. These nodes then coalesce laterally into a functional compact contractile ring. Although Mid1 is present at the medial cortex throughout G2 phase, recruitment of contractile ring components to nodes starts only at mitotic onset indicating that this event is cell cycle regulated. Polo kinases are key temporal coordinators of mitosis and cytokinesis and the Polo-like kinase Plo1 has long been implicated in Mid1 regulation: Plo1 activates Mid1 nuclear export at mitotic onset, coupling division plane specification to nuclear position. Here, we provide evidence that Plo1 also triggers the recruitment of contractile ring components into medial cortical nodes. Plo1 binds at least two independent sites on Mid1, including a consensus site phosphorylated by Cdc2. Plo1 phosphorylates several residues within the first 100 amino acids of Mid1, which directly interact with the IQGAP Rng2, and influences the timing of Myosin II recruitment. Plo1 thereby facilitates contractile ring assembly at mitotic onset.
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SFI1P具有保守的中心蛋白结合位点，并且在萌芽的酵母螺旋极体复制中具有重要功能。
影响因子：
7.8
作者：
Kilmartin, John V
通讯作者：
Kilmartin, John V
DOI：
10.1083/jcb.143.6.1603
发表时间：
1998-12-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
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通讯作者：
McCollum D
影响因子：
64.8
作者：
Moseley, James B.;Mayeux, Adeline;Nurse, Paul
通讯作者：
Nurse, Paul
DOI：
10.1083/jcb.200402097
发表时间：
2004-06-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Motegi F;Mishra M;Balasubramanian MK;Mabuchi I
通讯作者：
Mabuchi I
影响因子：
3.3
作者：
Mulvihill, DP;Petersen, J;Hagan, IM
通讯作者：
Hagan, IM