Diagnosis of Babesia caballi infections in horses by enzyme-linked immunosorbent assay (ELISA) and western blot
Diagnosis of Babesia caballi infections in horses by enzyme-linked immunosorbent assay (ELISA) and western blot
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DOI：
10.1016/0020-7519(94)90081-7
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发表时间：
1994-05
影响因子：
4
通讯作者：
Peymann B
中科院分区：
文献类型：
--
作者：
Böse R;Peymann B
从卡氏巴氏巴氏杆菌体外培养物中获得了100%感染红细胞的制剂。从这个，B。用去污剂3-[（3-胆酰胺丙基）-二甲基铵基]-1-丙烷磺酸盐（CHAPS）提取caballi抗原并用作ELISA抗原。以相同的方式制备来自相同供体马的正常红细胞的对照抗原。用B实验感染的马血清对ELISA和Western blot进行了验证。caballi或B。马或未感染巴贝虫属的马。将ELISA和Western blot结果与免疫荧光抗体试验（IFAT）和补体结合试验（CFT）进行比较。从感染后第14天开始，ELISA的敏感性为98.3%，上级Western blot（94.9%）、IFAT（96.6%）和CFT（28.8%）。用106份未感染巴伯氏丝虫的马血清进行ELISA和Western blot检测，均未获得阳性结果。导致两种测试的计算特异性为100%。B交叉反应。在ELISA中出现的等阳性血清（20%）比IFAT中出现的等阳性血清（4%）要多。与Western blot和CFT无交叉反应。通过对132份现场血清的检测也证明了ELISA的较高灵敏度：ELISA（112份）获得的阳性结果多于IFAT（92份）或CFT（41份）。这些结果的有效性通过Western blot检测血清得到证实。ELISA作为最敏感的检测方法，为识别带菌者马匹提供了最佳方法，以防止传入非流行地区（出口检测）。如果需要物种特异性诊断，则可以通过Western blot确认阳性ELISA结果。
From Babesia caballi in vitro cultures a preparation of 100% infected erythrocytes was obtained. From this, B. caballi antigens were extracted with the detergent 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and used as ELISA antigens. A control antigen of normal erythrocytes from the same donor horse was prepared in an identical manner. The ELISA and Western blot were validated by testing of sera from horses experimentally infected with B. caballi or B. equi or not infected with Babesia spp. ELISA and Western blot results were compared with those obtained by the immunofluorescence antibody test (IFAT) and complement fixation test (CFT). The sensitivity of the ELISA of 98.3% obtained for sera from day 14 after infection was superior to the Western blot (94.9%), the IFAT (96.6%) and the CFT (28.8%). No positive results were obtained in the ELISA and Western blot with 106 sera from horses not infected with Babesia spp. resulting in a calculated specificity of 100% for both tests. Cross reactions of B. equi-positive sera did occur to a larger extent in the ELISA (20%) than in the IFAT (4%). No cross reactions were observed with the Western blot and the CFT. The higher sensitivity of the ELISA was also demonstrated by testing of 132 field sera: more positive results were obtained by ELISA (112) as compared to IFAT (92) or CFT (41). The validity of these results was confirmed by testing of sera by Western blot. The ELISA as the most sensitive test provides the best method for the identification of carrier horses to prevent the introduction into non-endemic areas (export testing). Positive ELISA results can be confirmed by Western blot, if a species-specific diagnosis is required.
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影响因子：
56.9
作者：
LEVY, MG;RISTIC, M
通讯作者：
RISTIC, M
影响因子：
2
作者：
Böse R;Jacobson RH;Gale KR;Waltisbuhl DJ;Wright IG
通讯作者：
Wright IG
影响因子：
2.6
作者：
WEILAND, G
通讯作者：
WEILAND, G
影响因子：
9.4
作者：
BARLOUGH, JE;JACOBSON, RH;SCOTT, FW
通讯作者：
SCOTT, FW
影响因子：
4
作者：
BOSE, R;DAEMEN, K
通讯作者：
DAEMEN, K