Altered actin centripetal retrograde flow in physically restricted immunological synapses.
Altered actin centripetal retrograde flow in physically restricted immunological synapses.
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DOI：
10.1371/journal.pone.0011878
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发表时间：
2010-07-29
期刊：
影响因子：
3.7
通讯作者：
Groves JT
中科院分区：
文献类型：
--
作者：
Yu CH;Wu HJ;Kaizuka Y;Vale RD;Groves JT
T细胞的抗原识别涉及T细胞-抗原呈递细胞（APC）连接内大量受体、粘附和共刺激蛋白的大规模空间重组。由此产生的模式可以是独特的，并统称为免疫突触。细胞骨架网络的动态组装被认为在驱动这些组装过程中起着重要作用。在一种实验策略中，APC被合成的支撑膜代替。这种构造的优点在于，图案化到下面的基底上的固体结构可以引导免疫突触组装成改变的图案。在这里，我们在空间分配的支持双层上使用移动的抗CD 3 ε来连接和触发活Jurkat T细胞中的T细胞受体（TCR）。同时跟踪TCR簇和GFP-肌动蛋白斑点揭示了它们的动态关联和个体流动模式。肌动蛋白逆行流引导TCR簇的向内转运。基于流的粒子跟踪算法可以研究整个突触内肌动蛋白流场的速度分布，随着向突触中心移动，肌动蛋白流的向心速度逐渐减小。局部肌动蛋白流动分析表明，虽然没有直接从基板图案的肌动蛋白运动的影响，速度差异的肌动蛋白观察到物理上被困的TCR集群。肌动蛋白流在穿过受限的TCR簇后立即恢复其速度。这些观察结果与TCR簇和粘弹性肌动蛋白网络之间的动态和耗散耦合一致。
Antigen recognition by T cells involves large scale spatial reorganization of numerous receptor, adhesion, and costimulatory proteins within the T cell-antigen presenting cell (APC) junction. The resulting patterns can be distinctive, and are collectively known as the immunological synapse. Dynamical assembly of cytoskeletal network is believed to play an important role in driving these assembly processes. In one experimental strategy, the APC is replaced with a synthetic supported membrane. An advantage of this configuration is that solid structures patterned onto the underlying substrate can guide immunological synapse assembly into altered patterns. Here, we use mobile anti-CD3ε on the spatial-partitioned supported bilayer to ligate and trigger T cell receptor (TCR) in live Jurkat T cells. Simultaneous tracking of both TCR clusters and GFP-actin speckles reveals their dynamic association and individual flow patterns. Actin retrograde flow directs the inward transport of TCR clusters. Flow-based particle tracking algorithms allow us to investigate the velocity distribution of actin flow field across the whole synapse, and centripetal velocity of actin flow decreases as it moves toward the center of synapse. Localized actin flow analysis reveals that, while there is no influence on actin motion from substrate patterns directly, velocity differences of actin are observed over physically trapped TCR clusters. Actin flow regains its velocity immediately after passing through confined TCR clusters. These observations are consistent with a dynamic and dissipative coupling between TCR clusters and viscoelastic actin network.
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影响因子：
3.4
作者：
DeMond, Andrew L.;Mossman, Kaspar D.;Groves, Jay T.
通讯作者：
Groves, Jay T.
影响因子：
56.9
作者：
Hu, Ke;Ji, Lin;Waterman-Storer, Clare M.
通讯作者：
Waterman-Storer, Clare M.
影响因子：
64.8
作者：
Monks, CRF;Freiberg, BA;Kupfer, A
通讯作者：
Kupfer, A
DOI：
10.1073/pnas.0902621106
发表时间：
2009-08-04
影响因子：
11.1
作者：
Hartman, Nina C.;Nye, Jeffrey A.;Groves, Jay T.
通讯作者：
Groves, Jay T.
影响因子：
30.5
作者：
Barda-Saad, M;Braiman, A;Samelson, LE
通讯作者：
Samelson, LE