Metabolic adaptation to a high-fat diet is associated with a change in the gut microbiota.
Metabolic adaptation to a high-fat diet is associated with a change in the gut microbiota.
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DOI：
10.1136/gutjnl-2011-301012
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发表时间：
2012-04
期刊：
影响因子：
24.5
通讯作者：
Burcelin R
中科院分区：
文献类型：
--
作者：
Serino M;Luche E;Gres S;Baylac A;Bergé M;Cenac C;Waget A;Klopp P;Iacovoni J;Klopp C;Mariette J;Bouchez O;Lluch J;Ouarné F;Monsan P;Valet P;Roques C;Amar J;Bouloumié A;Théodorou V;Burcelin R
肠道微生物群被认为是代谢疾病的致病因素，在动物中表现得最好，它受到宿主基因组和营养环境的双重影响。这项研究调查了肠道微生物群本身，除了遗传背景和饮食的变化，是否可以在小鼠中标记不同的代谢表型。使用代谢适应的独特动物模型，其中喂食高脂肪无碳水化合物饮食（HFD）的C57 B1/6雄性小鼠变得患有糖尿病（HFD糖尿病，HFD-D）或抵抗糖尿病（HFD糖尿病抵抗，HFD-DR）。进行肠道微生物群的焦磷酸测序以分析不同代谢表型的肠道微生物群落。研究了炎症、肠道通透性、白色脂肪组织、肝脏和骨骼肌的特征。此外，为了直接改变肠道微生物群，另外一组小鼠被给予葡萄糖低聚糖（GOS）补充的HFD（HFD+GOS）。尽管小鼠具有相同的遗传背景和营养状况，但确定了对每种代谢表型特异性的肠道微生物谱。HFD-D肠道微生物特征与肠道通透性增加相关，肠道通透性增加与内毒素血症增加和内脏白色脂肪组织基质血管部分中细胞数量急剧增加相关。当肠道微生物群被GOS膳食纤维有意地改变时，HFD喂养的小鼠的大多数生理特征被调节。肠道微生物群是代谢表型的标志，与宿主遗传背景和饮食的差异无关。
The gut microbiota, which is considered a causal factor in metabolic diseases as shown best in animals, is under the dual influence of the host genome and nutritional environment. This study investigated whether the gut microbiota per se, aside from changes in genetic background and diet, could sign different metabolic phenotypes in mice. The unique animal model of metabolic adaptation was used, whereby C57Bl/6 male mice fed a high-fat carbohydrate-free diet (HFD) became either diabetic (HFD diabetic, HFD-D) or resisted diabetes (HFD diabetes-resistant, HFD-DR). Pyrosequencing of the gut microbiota was carried out to profile the gut microbial community of different metabolic phenotypes. Inflammation, gut permeability, features of white adipose tissue, liver and skeletal muscle were studied. Furthermore, to modify the gut microbiota directly, an additional group of mice was given a gluco-oligosaccharide (GOS)-supplemented HFD (HFD+GOS). Despite the mice having the same genetic background and nutritional status, a gut microbial profile specific to each metabolic phenotype was identified. The HFD-D gut microbial profile was associated with increased gut permeability linked to increased endotoxaemia and to a dramatic increase in cell number in the stroma vascular fraction from visceral white adipose tissue. Most of the physiological characteristics of the HFD-fed mice were modulated when gut microbiota was intentionally modified by GOS dietary fibres. The gut microbiota is a signature of the metabolic phenotypes independent of differences in host genetic background and diet.
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影响因子：
7.7
作者：
Cani, Patrice D.;Amar, Jacques;Burcelin, Remy
通讯作者：
Burcelin, Remy
影响因子：
3.1
作者：
Burcelin, Remy;Luche, Elodie;Amar, Jacques
通讯作者：
Amar, Jacques
DOI：
10.1152/ajpendo.00332.2001
发表时间：
2002-04-01
影响因子：
5.1
作者：
Burcelin, M;Crivelli, V;Thorens, B
通讯作者：
Thorens, B
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
24.5
作者：
Cani PD;Possemiers S;Van de Wiele T;Guiot Y;Everard A;Rottier O;Geurts L;Naslain D;Neyrinck A;Lambert DM;Muccioli GG;Delzenne NM
通讯作者：
Delzenne NM