Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats
Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats
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褪黑素对大鼠四氯化碳肝毒性的保护作用
DOI：
10.1002/cbf.1160
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发表时间：
2005
影响因子：
3.6
通讯作者：
R. Reiter
中科院分区：
文献类型：
--
作者：
L. Zavodnik;I. Zavodnik;E. Lapshina;E. Belonovskaya;D. I. Martinchik;R. Kravchuk;M. Bryszewska;R. Reiter
褪黑激素是一种吲哚胺，主要由松果体分泌到哺乳动物的血液中。本研究旨在探讨褪黑素对四氯化碳（CCl 4）诱导的大鼠急性肝损伤的保护作用。CCl 4通过产生自由基发挥其毒性作用;在将雄性Wistar大鼠（4 g kg−1体重）断头前20小时，将其灌胃给药。褪黑激素（15 mg kg−1体重）腹腔注射三次：注射CCl 4前30分钟和注射CCl 4后2 h和4 h。单独注射CCl 4的大鼠表现出显著的肝脏脂质和水肿性营养不良，肝细胞大量坏死，游离和结合胆红素水平显著增加，血浆中肝酶（丙氨酸氨基转移酶和天冬氨酸氨基转移酶）升高，以及肝脏和血液中NO蓄积。以药理剂量给予褪黑激素减少了CCl 4的毒性作用。因此，它减少了肝细胞的结构和功能损伤，具有明显的肝保护作用。褪黑激素给药也减少了CCl 4诱导的NO生成。这些结果表明，褪黑素对四氯化碳诱导的急性肝损伤的影响取决于褪黑素的抗氧化作用。版权所有© 2004年约翰威利父子有限公司。
Melatonin is an indolamine, mainly secreted by the pineal gland into the blood of mammalian species. The potential for protective effects of melatonin on carbon tetrachloride (CCl4)‐induced acute liver injury in rats was investigated in this work. CCl4 exerts its toxic effects by generation of free radicals; it was intragastrically administered to male Wistar rats (4 g kg−1 body weight) at 20 h before the animals were decapitated. Melatonin (15 mg kg−1 body weight) was administered intraperitoneally three times: 30 min before and at 2 and 4 h after CCl4 injection. Rats injected with CCl4 alone showed significant lipid and hydropic dystrophy of the liver, massive necrosis of hepatocytes, marked increases in free and conjugated bilirubin levels, elevation of hepatic enzymes (alanine aminotransferase and aspartate aminotransferase) in plasma, as well as NO accumulation in liver and in blood. Melatonin administered at a pharmacological dose diminished the toxic effects of CCl4. Thus it decreased both the structural and functional injury of hepatocytes and clearly exerted hepatoprotective effects. Melatonin administration also reduced CCl4‐induced NO generation. These findings suggest that the effect of melatonin on CCl4‐induced acute liver injury depends on the antioxidant action of melatonin. Copyright © 2004 John Wiley & Sons, Ltd.
影响因子：
2.9
作者：
GREEN, LC;WAGNER, DA;TANNENBAUM, SR
通讯作者：
TANNENBAUM, SR
影响因子：
--
作者：
E. Sewerynek;R. Reiter;D. Melchiorri;G. Ortiz;A. Lewiński
通讯作者：
E. Sewerynek;R. Reiter;D. Melchiorri;G. Ortiz;A. Lewiński