Morphogenesis and Organogenesis in the Regenerating Planktotrophic Larvae of Asteroids and Echinoids
Morphogenesis and Organogenesis in the Regenerating Planktotrophic Larvae of Asteroids and Echinoids
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小行星和海胆再生浮游营养幼虫的形态发生和器官发生
DOI：
10.2307/1543381
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
J. McClintock
中科院分区：
文献类型：
--
作者：
M. S. Vickery;M. Vickery;J. McClintock
在之前的一项研究中，我们描述了浮游营养小行星Luidia foliolata和Pisaster ochraceus的幼虫在手术切分后的完全身体再生（包括器官发生）。在这里，我们提出进一步详细观察这些独特的再生过程没有提出在以前的论文。此外，我们还首次报道了常规棘球蚴（Lytechinus variegatus）和不规则棘球蚴（Dendraster excentricus）浮游营养幼虫在手术切开后的完全再生。不论发育阶段如何，小行星和棘足类幼虫均表现出快速再生的能力。切分后48 h，手术切分部位可见假足间充质细胞聚集。这些细胞聚集在外观上类似于成年棘皮动物在手臂再生之前形成的间充质母细胞，也类似于其他后口动物在再生过程中所描述的细胞聚集。当小行星幼虫在发育早期被手术切开时，成簇的间充质细胞发育成位于新再生消化道前方的全新的体腔囊。这表明再生小行星幼虫的细胞命运在早期发育过程中仍然是不确定的。不论剖分时的发育阶段如何，鱼的前肢和后肢都能再生所有缺失的器官和组织。然而，在双翅期后期，小叶扁豆的两半幼虫表现出不同的再生能力。观察到的差异可能是由于幼虫发育的差异（L. foliolata没有臂线虫阶段），并可能具有进化意义。在正常的棘球蚴中，两个幼虫部分都再生为形态和功能正常的幼虫，与未分割的对照幼虫没有区别。再生过程与我们在浮游营养小行星幼虫中观察到的相似。再生的幼虫很容易蜕变成正常的幼体。在2周的研究中，不规则棘球蚴幼虫的后部完成了再生和变态，但前部仅部分再生。我们的观察证实，小行星和棘刺类幼虫为研究后口动物的再生提供了很好的模型。
In a previous study, we described complete body regeneration (with organogenesis) following surgical bisection in the planktotrophic larvae of the asteroids Luidia foliolata and Pisaster ochraceus. Here we present further detailed observations of these unique regenerative processes not presented in the previous paper. Furthermore, we describe for the first time complete regeneration following surgical bisection of planktotrophic larvae of the regular echinoid Lytechinus variegatus and the irregular echinoid Dendraster excentricus. Larvae of both asteroids and echinoids displayed a capacity for rapid regeneration regardless of their developmental stage. Within 48 h after bisection, aggregations of mesenchyme cells with pseudopodia were observed at the site of surgical bisection. These cellular aggregations were similar in appearance to the mesenchymal blastemas that form in adult echinoderms prior to their arm regeneration, and to those described in other deuterostomes that undergo regeneration. When asteroid larvae were surgically bisected in the early stages of their development, clusters of mesenchyme cells developed into completely new pairs of coelomic pouches located anterior to the newly regenerated digestive tract. This indicates that cell fate in regenerating asteroid larvae remains indeterminate during early development. In the larvae of P. ochraceus, regardless of the developmental stage at the time of bisection, both the anterior and posterior portions regenerated all their missing organs and tissues. However, the larvae of L. foliolata displayed differential regenerative capacity in bisected larval halves at the late bipinnaria stage. The differences observed may be due to differences in larval development (L. foliolata has no brachiolaria stage), and may have evolutionary implications. In the regular echinoid L. variegatus, both larval portions regenerated into morphologically and functionally normal larvae that were indistinguishable from non-bisected control larvae. The regenerative processes were similar to those we observed in planktotrophic asteroid larvae. Regenerating larvae readily metamorphosed into normal juveniles. In the irregular echinoid D. excentricus, posterior portions of larvae completed regeneration and metamorphosis, but anterior portions regenerated only partially during the 2-week study. Our observations confirm that asteroid and echinoid larvae provide excellent models for studies of regeneration in deuterostomes.
影响因子：
4
作者：
Peterson, KJ;Cameron, RA;Davidson, EH
通讯作者：
Davidson, EH
海胆发育过程中的细胞类型规范。
DOI：
10.1016/0168-9525(91)90367-y
发表时间：
1991
期刊：
Trends in genetics : TIG
影响因子：
--
作者：
Cameron,RA;Davidson,EH
通讯作者：
Davidson,EH