Comparative stimulation of motilin duodenal receptor by porcine or canine motilin.
Comparative stimulation of motilin duodenal receptor by porcine or canine motilin.
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猪或犬胃动素对胃动素十二指肠受体的比较刺激。
DOI：
10.1016/0016-5085(87)90014-x
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发表时间：
1987
期刊：
影响因子：
29.4
通讯作者：
L. Trudel
中科院分区：
文献类型：
--
作者：
P. Poitras;R. Lahaie;S. St.;L. Trudel
从猪和犬肠道纯化的胃动素在7 - 8 - 12 - 13 - 14位的氨基酸组成上不同。我们在体外研究了不同动物（豚鼠、兔、狗）的十二指肠纵行肌对猪和犬合成胃动素的收缩反应。这两种物质均未能引起豚鼠十二指肠收缩，但对家兔肌肉具有活性和同等效力。在犬中，猪胃动素在试验浓度（高达10 − 4 M）下无活性，而犬胃动素以剂量反应方式诱导十二指肠收缩（诱导半数最大反应所需的平均剂量：4.82 ± 0.25 × 10 − 5 M）。合成犬胃动素（10 − 5 M）产生的收缩不受阿托品、六甲双铵、河豚毒素、纳洛酮或硝普钠（均使用10 − 4 M）的影响，但可被维拉帕米（10 − 4）阻断。我们的研究（a）表明胃动素分子中与物种相关的结构改变在某些动物物种中产生不同的生物活性能力，（B）表明该分子的中间部分对其生物活性表达很重要，（c）表明犬十二指肠肌肉上存在胃动素受体，和（d）表明细胞外钙的内流涉及由犬胃动素引起的犬十二指肠肌肉收缩。
Motilins purified from porcine and canine intestine differ in their amino acid composition in positions 7-8-12-13-14. We studied in vitro the contractile response of longitudinal duodenal muscles from various animals (guinea pig, rabbit, dog) to porcine and canine synthetic motilins. Both substances failed to elicit contraction of the guinea pig duodenum but were active and equally potent on rabbit muscle. In dogs, porcine motilin was inactive at the concentrations tested (up to 10−4M) whereas canine motilin induced duodenal contractions in a doseresponse fashion (mean dose required to induce half-maximal response: 4.82 ± 0.25 × 10−5M). The contraction generated by synthetic canine motilin (10−5M) was not influenced by atropine, hexamethonium, tetrodotoxin, naloxone, or sodium nitroprusside (all used at 10−4M) but was blocked by verapamil (10−4). Our study (a) shows that species-related structural alterations in motilin molecules generate different bioactive capacities in some animal species, (b) suggests that the middle portion of the molecule is important for its bioactive expression, (c) suggests the presence of motilin receptors on canine duodenal muscle, and (d) suggests that an influx of extracellular calcium is involved in the canine duodenal muscle contraction elicited by canine motilin.
犬肠胃动素的快速高产纯化及其全序列测定
DOI：
10.1016/s0021-9673(01)90460-1
发表时间：
1985
期刊：
Journal of chromatography
影响因子：
--
作者：
ReeveJr,JR;Ho,FJ;Walsh,JH;Ben-Avram,CM;Shively,JE
通讯作者：
Shively,JE