Low dose theophylline showed an inhibitory effect on the production of IL-6 and IL-8 in primary lung fibroblast from patients with COPD.
Low dose theophylline showed an inhibitory effect on the production of IL-6 and IL-8 in primary lung fibroblast from patients with COPD.
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低剂量茶碱对 COPD 患者原代肺成纤维细胞产生 IL-6 和 IL-8 显示出抑制作用
DOI：
10.1155/2012/492901
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发表时间：
2012
影响因子：
4.6
通讯作者：
Qu JM
中科院分区：
文献类型：
--
作者：
Zhang J;Feng MX;Qu JM
慢性阻塞性肺疾病(COPD)是以肺部异常炎症和慢性炎症为特征的疾病。我们假设肺成纤维细胞可能参与了局部炎症，并研究了小剂量茶碱是否对成纤维细胞炎症有有益影响。入选接受肺癌肺叶切除术的患者，根据肺活量测定将其分为COPD组和对照组。原代培养的人肺成纤维细胞取自远离肿瘤组织的外周肺组织。COPD组成纤维细胞中IL-6和IL-8的表达和蛋白水平均显著升高，且与肺功能呈负相关(P<；0.05)。经5 g/m L茶碱处理后，IL-6和IL-8的蛋白水平分别从993.0±738.9 pg/m l和703.1±278.0 pg/m l降至650.1±421.9 pg/m l(P=0.014)和492.0±214.9 μp g/m l(P=0.001)。此外，5 μg/mL5 μg/mL茶碱可抑制1 mLLPS诱导的原代人肺成纤维细胞产生IL-6和IL-8。提示肺成纤维细胞通过释放IL-6和IL-8参与慢性阻塞性肺疾病的慢性炎症反应，小剂量茶碱可减轻成纤维细胞产生的促炎介质。
Chronic obstructive pulmonary disease (COPD) is characterized by the abnormal and chronic lung inflammation. We hypothesized that lung fibroblasts could contribute to the local inflammation and investigated whether low dose theophylline had a beneficial effect on fibroblasts inflammation. Subjects undergoing lobectomy for bronchial carcinoma were enrolled and divided into COPD and control groups according to spirometry. Primary human lung fibroblasts were cultured from peripheral lung tissue distant to tumor tissue. There was a significant increase in both the mRNA expressions and protein levels for IL-6 and IL-8 in fibroblasts in COPD group, and the values were negatively correlated with lung function (P < 0.05). For COPD fibroblasts, the protein levels of IL-6 and IL-8 decreased from 993.0 ± 738.9 pg/mL to 650.1 ± 421.9 pg/mL (P = 0.014) and from 703.1 ± 278.0 pg/mL to 492.0 ± 214.9 pg/mL (P = 0.001), respectively, with 5 μg/mL theophylline treatment. In addition, theophylline at the dose of 5 μg/mL reduced the increased production of IL-6 and IL-8 induced by 1 μg/mL LPS in primary human lung fibroblasts. Our data suggest that lung fibroblasts participate in the chronic inflammation in COPD by releasing IL-6 and IL-8, and low dose theophylline can alleviate the proinflammatory mediators' production by fibroblasts.
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组蛋白脱乙酰基酶2介导的糖皮质激素受体的脱乙酰基化可以抑制NF-kappab。
影响因子：
15.3
作者：
Ito, Kazuhiro;Yamamura, Satoshi;Essilfie-Quaye, Sarah;Cosio, Borja;Ito, Misako;Barnes, Peter J;Adcock, Ian M
通讯作者：
Adcock, Ian M
影响因子：
82.9
作者：
Fink, L;Seeger, W;Bohle, RM
通讯作者：
Bohle, RM
茶碱恢复了COPD巨噬细胞中组蛋白脱乙酰基酶的活性和类固醇反应。
影响因子：
15.3
作者：
Cosio, BG;Tsaprouni, L;Ito, K;Jazrawi, E;Adcock, IM;Barnes, PJ
通讯作者：
Barnes, PJ
DOI：
10.1513/pats.200504-024sr
发表时间：
2005-01-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Barnes, Peter J
通讯作者：
Barnes, Peter J
影响因子：
9.6
作者：
Ford, Paul A.;Durham, Andrew L.;Barnes, Peter J.
通讯作者：
Barnes, Peter J.