Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli.
Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli.
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DOI：
10.1038/s41598-018-26748-z
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发表时间：
2018-05-31
影响因子：
4.6
通讯作者：
Sugimoto S
中科院分区：
文献类型：
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作者：
Arita-Morioka KI;Yamanaka K;Mizunoe Y;Tanaka Y;Ogura T;Sugimoto S
生物膜是嵌入在自产的细胞外基质中的组织良好的微生物群落（例如，卷曲淀粉样纤维）并与慢性感染有关。因此，开发抗生物膜药物对于对抗这些感染是重要的。先前，我们发现黄酮醇杨梅素抑制大肠杆菌卷曲依赖性生物膜形成（IC 50 = 46.2 μM）。在这项研究中，我们测试了七种杨梅素衍生物抑制E. coli K-12液体培养。其中，只有表没食子儿茶素没食子酸酯（EGCG），一种主要的儿茶素在绿色茶，抑制K-12的生物膜形成（IC 50 = 5.9 μM）比杨梅有效。透射电子显微镜和免疫印迹分析表明，表没食子儿茶素没食子酸酯通过抑制卷曲相关蛋白的表达来防止卷曲的产生。定量RT-PCR分析表明，在EGCG的存在下，csgA，csgB和csgD的转录显着减少。有趣的是，细胞水平的RpoS，一个静止期特异性的替代σ因子，减少在存在的EGCG，而rpoS转录不受影响。抗生素追踪实验和遗传分析表明，EGCG加速了ATP依赖性蛋白酶ClpXP与其衔接子RssB的结合对RpoS的降解。总的来说，这些结果为治疗慢性生物膜相关感染的药物开发提供了重要的见解。
Biofilms are well-organised communities of microbes embedded in a self-produced extracellular matrix (e.g., curli amyloid fibers) and are associated with chronic infections. Therefore, development of anti-biofilm drugs is important to combat with these infections. Previously, we found that flavonol Myricetin inhibits curli-dependent biofilm formation by Escherichia coli (IC50 = 46.2 μM). In this study, we tested activities of seven Myricetin-derivatives to inhibit biofilm formation by E. coli K-12 in liquid culture. Among them, only Epigallocatechin gallate (EGCG), a major catechin in green tea, inhibited biofilm formation of K-12 (IC50 = 5.9 μM) more efficiently than Myricetin. Transmission electron microscopy and immunoblotting analyses demonstrated that EGCG prevented curli production by suppressing the expression of curli-related proteins. Quantitative RT-PCR analysis revealed that the transcripts of csgA, csgB, and csgD were significantly reduced in the presence of EGCG. Interestingly, the cellular level of RpoS, a stationary-phase specific alternative sigma factor, was reduced in the presence of EGCG, whereas the rpoS transcript was not affected. Antibiotic-chase experiments and genetic analyses revealed that EGCG accelerated RpoS degradation by ATP-dependent protease ClpXP in combination with its adaptor RssB. Collectively, these results provide significant insights into the development of drugs to treat chronic biofilm-associated infections.
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影响因子：
3.6
作者：
Nenninger AA;Robinson LS;Hammer ND;Epstein EA;Badtke MP;Hultgren SJ;Chapman MR
通讯作者：
Chapman MR
影响因子：
6.7
作者：
Kai-Larsen Y;Lüthje P;Chromek M;Peters V;Wang X;Holm A;Kádas L;Hedlund KO;Johansson J;Chapman MR;Jacobson SH;Römling U;Agerberth B;Brauner A
通讯作者：
Brauner A
DOI：
10.1073/pnas.0812143106
发表时间：
2009-01-20
影响因子：
11.1
作者：
Nenninger, Ashley A.;Robinson, Lloyd S.;Hultgren, Scott J.
通讯作者：
Hultgren, Scott J.
影响因子：
14.8
作者：
Cegelski, Lynette;Pinkner, Jerome S.;Hammer, Neal D.;Cusumano, Corinne K.;Hung, Chia S.;Chorell, Erik;Aberg, Veronica;Walker, Jennifer N.;Seed, Patrick C.;Almqvist, Fredrik;Chapman, Matthew R.;Hultgren, Scott J.
通讯作者：
Hultgren, Scott J.
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
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