Molecular Characterization of Mg-Chelatase CHLI Subunit in Pea (Pisum sativum L.).
Molecular Characterization of Mg-Chelatase CHLI Subunit in Pea (Pisum sativum L.).
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豌豆 (Pisum sativum L.) 中镁螯合酶 CHLI 亚基的分子表征
DOI：
10.3389/fpls.2022.821683
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发表时间：
2022
影响因子：
5.6
通讯作者：
Luo S
中科院分区：
文献类型：
--
作者：
Wu CJ;Wang J;Zhu J;Ren J;Yang YX;Luo T;Xu LX;Zhou QH;Xiao XF;Zhou YX;Luo S
镁螯合酶作为叶绿素合成的限速酶，是提高光合效率的一个很有前途的靶酶。它由CHLH、CHLD和CHLI亚基组成。在豌豆（Pisum sativum L.）中，最近通过全基因组测序揭示了两个推定的CHLI基因（PsCHLI 1和PsCHLI 2），但它们的分子特征还没有完全表征。在这项研究中，PsCHLI 1和PsCHLI 2的cDNA鉴定基于PCR的克隆和测序。系统发育分析表明，PsCHLIs来自一个古老的复制豆类。这两个PsCHLIs更高的表达在叶片中比在其他器官和下调脱落酸和热处理，而PsCHLI 1更高的表达比PsCHLI 2。PsCHLI 1和PsCHLI 2分别编码422和417个氨基酸的蛋白质，它们具有82%的氨基酸同源性，并且位于叶绿体中。与沉默的PsCHLI 1和PsCHLI 2双沉默的植物非常相似的植物，都表现出黄色的叶子几乎检测不到镁螯合酶活性和叶绿素含量。此外，具有沉默的PsCHLI 2的植物没有表现出明显的表型。此外，PsCHLI 1的N端片段（PsCHLI 1 N，Val 63-Cys 191）和中间片段（PsCHLI 1 M，Gly 192-Ser 336）分别介导同源二聚体的形成和与CHLD的相互作用，而活性PsCHLI 1仅通过PsCHLI 1 N，PsCHLI 1 M和PsCHLI 1的C端片段（Ser 337-Ser 422）结合来实现。综上所述，PsCHLI 1是关键的CHLI亚基，其肽段对于维持镁螯合酶活性是必需的，这可以用于通过操纵豌豆中的镁螯合酶来提高光合效率。
As a rate-limiting enzyme for chlorophyll biosynthesis, Mg-chelatase is a promising target for improving photosynthetic efficiency. It consists of CHLH, CHLD, and CHLI subunits. In pea (Pisum sativum L.), two putative CHLI genes (PsCHLI1 and PsCHLI2) were revealed recently by the whole genome sequencing, but their molecular features are not fully characterized. In this study, PsCHLI1 and PsCHLI2 cDNAs were identified by PCR-based cloning and sequencing. Phylogenetic analysis showed that PsCHLIs were derived from an ancient duplication in legumes. Both PsCHLIs were more highly expressed in leaves than in other organs and downregulated by abscisic acid and heat treatments, while PsCHLI1 was more highly expressed than PsCHLI2. PsCHLI1 and PsCHLI2 encode 422- and 417-amino acid proteins, respectively, which shared 82% amino acid identity and were located in chloroplasts. Plants with a silenced PsCHLI1 closely resembled PsCHLI1 and PsCHLI2 double-silenced plants, as both exhibited yellow leaves with barely detectable Mg-chelatase activity and chlorophyll content. Furthermore, plants with a silenced PsCHLI2 showed no obvious phenotype. In addition, the N-terminal fragment of PsCHLI1 (PsCHLI1N, Val63-Cys191) and the middle fragment of PsCHLI1 (PsCHLI1M, Gly192-Ser336) mediated the formation of homodimers and the interaction with CHLD, respectively, while active PsCHLI1 was only achieved by combining PsCHLI1N, PsCHLI1M, and the C-terminal fragment of PsCHLI1 (Ser337-Ser422). Taken together, PsCHLI1 is the key CHLI subunit, and its peptide fragments are essential for maintaining Mg-chelatase activity, which can be used to improve photosynthetic efficiency by manipulating Mg-chelatase in pea.
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影响因子：
7.4
作者：
Guo, RB;Luo, MZ;Weinstein, JD
通讯作者：
Weinstein, JD
DOI：
10.1111/j.1432-1033.2004.04143.x
发表时间：
2004-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Lake, V;Olsson, U;Hansson, M
通讯作者：
Hansson, M
影响因子：
4.1
作者：
Jensen, PE;Gibson, LCD;Hunter, CN
通讯作者：
Hunter, CN
硫氧还蛋白氧化还原调节镁螯合酶 CHLI 亚基的 ATP 酶活性并调节豌豆植物四吡咯生物合成和活性氧稳态中氧化还原介导的信号传导
影响因子：
7.4
作者：
Luo, Tao;Fan, Tingting;Luo, Meizhong
通讯作者：
Luo, Meizhong
影响因子：
14.8
作者：
Croce, Roberta;van Amerongen, Herbert
通讯作者：
van Amerongen, Herbert