In vivo imaging of Ca2+ accumulation during cotton fiber initiation using fluorescent indicator YC3.60
In vivo imaging of Ca2+ accumulation during cotton fiber initiation using fluorescent indicator YC3.60
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使用荧光指示剂 YC3.60 对棉纤维起始过程中 Ca2 积累的体内成像
DOI：
10.1007/s00299-017-2122-3
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发表时间：
2017-03
影响因子：
6.2
通讯作者：
Yan Pei
中科院分区：
文献类型：
--
作者：
Lei Hou;hui-qing Song;Jianyan Zeng;Yan Pei
Non-tip-focused Ca2+gradient indicated by genetically expressing a FRET-based calcium sensor YC3.60 was established in spherical expanding cotton fibers, which is vital for cotton fiber initiation. Cotton fiber is a single cell elongated from ovule epidermis. It is not only the most important natural fiber used in the textile industry but also an ideal model for studying cell differentiation and elongation. Before linear cell growth, cotton fibers undergo spherical expansion at the beginning of initiation. Ca2+, as an important secondary messenger, plays a central role in polarized cell growth including cotton fiber elongation. However, the role of Ca2+in fiber initiation is far from well understood. In this paper, through ovule culture we demonstrate that Ca2+is crucial for fiber initiation. Using transgenic cotton expressing the fluorescent Ca2+indicator YC3.60, we show cellular and intracellular distribution of Ca2+in cotton ovule epidermis and fiber cells. In the initiating fiber cell, Ca2+accumulated mainly at the base of the cell, while in the fast elongating cell, the Ca2+was enriched in the tip region. This cellular distribution of Ca2+reported by YC3.60 was confirmed by the staining with a Ca2+-sensitive dye fluo-3/AM. Compared to the fluorescent dye staining, the YC3.60 system can reveal more detailed information on the intracellular distribution without photobleaching. Taken together, our data suggest that Ca2+plays an important role in spherical expansion of cotton fiber initials.
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影响因子：
4.4
作者：
Padmalatha KV;Patil DP;Kumar K;Dhandapani G;Kanakachari M;Phanindra ML;Kumar S;Mohan TC;Jain N;Prakash AH;Vamadevaiah H;Katageri IS;Leelavathi S;Reddy MK;Kumar PA;Reddy VS
通讯作者：
Reddy VS
影响因子：
4.3
作者：
J. Schiefelbein;A. Shipley;Paul Rowse
通讯作者：
J. Schiefelbein;A. Shipley;Paul Rowse
DOI：
10.1101/000745
发表时间：
2013-11
期刊：
bioRxiv
影响因子：
--
作者：
M. Pang;N. Sanford;T. Wilkins
通讯作者：
M. Pang;N. Sanford;T. Wilkins
DOI：
--
发表时间：
1999
期刊：
--
影响因子：
--
作者：
A. Basra
通讯作者：
A. Basra
影响因子：
--
作者：
P. He;Libo Shan;J. Sheen
通讯作者：
P. He;Libo Shan;J. Sheen