The extracts of Japanese willow tree species are effective forapoptotic desperation or differentiation of acute myeloid leukemia cells.
The extracts of Japanese willow tree species are effective forapoptotic desperation or differentiation of acute myeloid leukemia cells.
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DOI：
10.4103/0973-1296.131023
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发表时间：
2014-04
影响因子：
0.7
通讯作者：
Fujita T
中科院分区：
文献类型：
--
作者：
Fujita K;Nomura Y;Sawajiri M;Mohapatra PK;El-Shemy HA;Nguyen NT;Hosokawa M;Miyashita K;Maeda T;Saneoka H;Fujita S;Fujita T
研究了生长在不同氮素水平下的几种日本柳树植物部分热水提取物的抗白血病活性。本研究制备了不同氮素营养水平下的柳叶水提物。叶和茎提取物具有显著的抗白血病活性。幼叶、成熟叶和茎的热水粗提物在孵育48h后对急性髓系白血病(AML)细胞(HL60和NB4)有明显的杀灭作用，而根部提取液对AML细胞(HL60和NB4)的杀伤力较小。与植物部分相似，不同柳树种提取物的反应也不相同，不同树种间的死亡细胞占整个培养基细胞的比例在21%~93%之间。在较高的氮素营养水平下，反应灵敏的柳树树种的叶提取物降低了活细胞百分率，增加了死亡细胞百分率。经异硫氰酸荧光素(FITC)标记的Annexin V标记的AML细胞经叶提取物处理后，细胞出现明显的细胞凋亡。当无反应的AML细胞提取物加入培养液中时，AML细胞的分化仍未减弱，细胞凋亡率较低。
The antileukemic activity of hot water extract of plant parts of some Japanese willow tree species grown at different levels of nitrogen were examined. Water extracts of willow leaves were prepared for this studies in different level of nitrogen nutrition. The extracts obtained from the leaves and stem exhibited anti-leukemic activities prominently. The crude hot water extracts of the young growing parts including apex, matured leaves and stem, killed the blasts of acute myeloid leukemia (AML) cells, (HL60 and NB4) after 48h incubation, however, such desperation was far less in the root extract. Similar to the plant parts, response of extracts obtained from different willow species was not identical; the proportion of dead cells relative to whole cells of the culture medium ranged from 21% to 93% among the species. Leaf extracts obtained from the responsive willow species decreased the live cell percentage and increased the dead cell percentage at higher level of nitrogen nutrition. The mode of desperation of leaf extract treated AML cells in such species appeared to be cell apoptosis as shown by binding with fluorescein isothiocyanate (FITC) -labeled Annexin V. Differentiation of alive AML cells continued unabated and apoptosis was poor when extract of an unresponsive species added to the culture medium.
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影响因子：
6
作者：
Wilkinson, AG
通讯作者：
Wilkinson, AG
影响因子：
8.5
作者：
Al-Abd, A. M.;Mahmoud, A. M.;El-Shemy, H. A.
通讯作者：
El-Shemy, H. A.
DOI：
10.1016/j.bbagen.2004.08.012
发表时间：
2004-11-18
影响因子：
3
作者：
Hosokawa, M;Kudo, M;Miyashita, K
通讯作者：
Miyashita, K
DOI：
10.1016/j.cbpc.2005.10.005
发表时间：
2006-01-01
影响因子：
3.9
作者：
Konishi, I;Hosokawa, M;Miyashita, K
通讯作者：
Miyashita, K
DOI：
10.1007/978-94-007-0083-3_1
发表时间：
2011-01-01
期刊：
MULTIMEDIA MULTIPROCESSOR SYSTEMS: ANALYSIS, DESIGN AND MANAGEMENT
影响因子：
--
作者：
Kumar, Akash;Corporaal, Henk;Ha, Yajun
通讯作者：
Ha, Yajun