Investigations of the constitutive overexpression of CYP6D1 in the permethrin resistantLPR strain of house fly (Musca domestica).
Investigations of the constitutive overexpression of CYP6D1 in the permethrin resistantLPR strain of house fly (Musca domestica).
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DOI：
10.1016/j.pestbp.2011.02.012
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发表时间：
2011-06
影响因子：
4.7
通讯作者：
Scott JG
中科院分区：
文献类型：
--
作者：
Lin GG;Scott JG
家蝇（Musca acutitica）CYP 6D 1是一种参与外源性物质代谢的细胞色素P450。CYP 6D 1位于1号染色体上，在杀虫剂敏感菌株中，其表达可被原型P450诱导剂苯巴比妥（PB）诱导。CYP 6D 1的转录增加赋予LPR菌株对氯菊酯的抗性，并且该性状映射到1号和2号染色体。然而，LPR中CYP 6D 1的组成性过表达不会被PB进一步增加，并且对PB的无反应性映射到2号染色体。有研究表明，2号染色体上的一个单一因子可能是导致LPR中CYP 6D 1组成性过表达和PB诱导缺乏的原因。我们在果蝇S2细胞中使用双荧光素酶报告基因检测了LPR的CYP 6D 1v 1启动子的PB诱导，发现CYP 6D 1v 1启动子能够介导PB诱导，类似于杀虫剂敏感CS菌株的CYP 6D 1v 2启动子。因此，CYP 6D 1v 1和v2启动子序列的变异似乎与LPR中CYP 6D 1v 1的PB诱导缺乏无关;这表明一种未鉴定的反式作用因子负责。HR 96在黑腹果蝇和M.南极洲因此，家蝇HR 96 cDNA克隆和测序，以检查是否这种反式作用因子是负责组成型过表达的CYP 6D 1v 1在LPR。鉴定了多个HR 96等位基因（v1-v10），但均与抗性无关。HR 96在LPR和CS中的表达水平无差异。因此，HR 96不是负责LPR中CYP 6D 1组成性过表达的反式作用因子。这种反式作用因子的身份仍然难以捉摸。
House fly (Musca domestica) CYP6D1 is a cytochrome P450 involved in metabolism of xenobiotics. CYP6D1 is located on chromosome 1 and its expression is inducible in response to the prototypical P450 inducer phenobarbital (PB) in insecticide susceptible strains. Increased transcription of CYP6D1 confers resistance to permethrin in the LPR strain, and this trait maps to chromosomes 1 and 2. However, the constitutive overexpression of CYP6D1 in LPR is not further increased by PB and the non-responsiveness to PB maps to chromosome 2. It has been suggested that a single factor on chromosome 2 could be responsible for both the constitutive overexpression and lack of PB induction of CYP6D1 in LPR. We examined the PB inducibility of CYP6D1v1 promoter from LPR using dual luciferase reporter assays in Drosophila S2 cells and found the CYP6D1v1 promoter was able to mediate PB induction, similar to the CYP6D1v2 promoter from the insecticide susceptible CS strain. Therefore, variation in promoter sequences of CYP6D1v1 and v2 does not appear responsible for the lack of PB induction of CYP6D1v1 in LPR; this suggests an unidentified trans acting factor is responsible. HR96 has been implicated in having a role in PB induction in Drosophila melanogaster and M. domestica. Therefore, house fly HR96 cDNA was cloned and sequenced to examine if this trans acting factor is responsible for constitutive overexpression of CYP6D1v1 in LPR. Multiple HR96 alleles (v1–v10) were identified, but none were associated with resistance. Expression levels of HR96 were not different between LPR and CS. Thus, HR96 is not the trans acting factor responsible for the constitutive overexpression of CYP6D1 in LPR. The identity of this trans acting factor remains elusive.
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影响因子：
14.9
作者：
Rose, TM;Henikoff, JG;Henikoff, S
通讯作者：
Henikoff, S
影响因子：
4.7
作者：
HATANO, R;SCOTT, JG
通讯作者：
SCOTT, JG
影响因子：
2.2
作者：
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通讯作者：
Scott, JG
影响因子：
3.8
作者：
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通讯作者：
LEE, SST
影响因子：
3.8
作者：
TOMITA, T;SCOTT, JG
通讯作者：
SCOTT, JG