Microscopic observations of strawberry plant colonization by a GFP-labelled strain of Fusarium oxysporum f. sp. fragariae
Microscopic observations of strawberry plant colonization by a GFP-labelled strain of Fusarium oxysporum f. sp. fragariae
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GFP 标记的尖孢镰刀菌 (Fusarium oxysporum f) 菌株对草莓植株定植的显微镜观察。
DOI：
10.1080/07060661.2014.965218
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发表时间：
2014-10
影响因子：
2
通讯作者：
Zhang Baolong
中科院分区：
文献类型：
--
作者：
Yuan Hongbo;Ling Xitie;Liu Tingli;Chen Tianzi;Yang Yuwen;Yao Shu;Zhang Baolong
摘要尖孢镰刀菌f.草莓枯萎病病原菌Fragaria sp. fragariae，对商业生产是一个严重的威胁。了解草莓与枯萎病菌的互作关系。利用农杆菌介导的转化方法，以绿色荧光蛋白为标记基因，Hyg为选择标记基因，对草莓枯萎病菌进行了遗传转化。获得的转化子稳定，具有强且均匀的荧光。利用一株与野生型菌株具有相似表型和致病性的绿色荧光蛋白标记菌株对草莓进行侵染过程研究。结果表明，孢子从芽管附着于草莓根表，侵染草莓根，菌丝从菌丝顶端产生吸盘状结构，侵染草莓植株。一旦进入，菌丝生长主要在表皮和皮层组织中观察到，只有少数菌丝在定殖后的维管组织中检测到。
Abstract Fusarium oxysporum f. sp. fragariae, the causal agent of Fusarium wilt in strawberry (Fragaria ananassa Duch.), is a serious threat to commercial production. To understand the interaction between strawberry and Fusarium oxysporum f. sp. fragariae, we transformed the pathogen with a green fluorescent protein as a labelled gene and Hyg as a selectable marker gene using the Agrobacterium tumefaciens-mediated transformation method. The transformants obtained were stable with strong and uniform fluorescence. A green fluorescent protein-tagged strain with similar phenotype and pathogenicity as the wild-type strain was used to study the infection process on strawberry. The observations indicated that the spores attached to the root surface and infected the roots from germ tubes, while the hyphae produced suction cup-like structures from the hyphal tips to infect the strawberry plants. Once inside, hyphal growth was observed mainly in the epidermal and cortical tissues with only a few hyphae detected in the vascular tissues after colonization.
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DOI：
--
发表时间：
2010
期刊：
--
影响因子：
--
作者：
Gu ChunBo;Shi Xiaobin;Jiang Lili;Wang Kaiyun;Lin CaiHua;Du Haiming
通讯作者：
Gu ChunBo;Shi Xiaobin;Jiang Lili;Wang Kaiyun;Lin CaiHua;Du Haiming
DOI：
--
发表时间：
2006
期刊：
Journal of Sichuan Agricultural University
影响因子：
--
作者：
Ma Shu-qing
通讯作者：
Ma Shu-qing
影响因子：
1.2
作者：
T. Nonomura;H. Tajima;Y. Kitagawa;Naoko Sekiya;Kayoko Shitomi;Mami Tanaka;Kazuhiko Maeda;Y. Matsuda;H. Toyoda
通讯作者：
T. Nonomura;H. Tajima;Y. Kitagawa;Naoko Sekiya;Kayoko Shitomi;Mami Tanaka;Kazuhiko Maeda;Y. Matsuda;H. Toyoda
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
DOI：
--
发表时间：
2011
期刊：
Cotton Science
影响因子：
--
作者：
Zhang Bao-long
通讯作者：
Zhang Bao-long