Down-regulation of lipoprotein lipase increases ABCA1-mediated cholesterol efflux in THP-1 macrophages.
Down-regulation of lipoprotein lipase increases ABCA1-mediated cholesterol efflux in THP-1 macrophages.
复制标题
DOI：
10.1016/j.bbrc.2014.07.015
复制
发表时间：
2014-08-08
影响因子：
3.1
通讯作者：
Medh, Jheem D.
中科院分区：
文献类型：
--
作者：
Kawashima, Ryoko L.;Medh, Jheem D.
关键词：
ATP结合盒转运蛋白A1（ABCA 1）介导过量胆固醇从泡沫细胞流出到贫脂载脂蛋白A-I，这一过程称为胆固醇反向转运。脂蛋白脂酶（LPL）是由动脉粥样硬化病变内的巨噬细胞表达的脂解酶。慢病毒介导的RNA干扰用于基因敲低（KD）THP-1巨噬细胞中LPL的表达。通过终点PCR、真实的时间PCR和蛋白质分析证实LPL基因的沉默。LPL表达的抑制与ABCA 1 mRNA水平的1.6倍上调相关，并导致ABCA 1依赖性胆固醇流出增加4.5倍。通过向细胞培养基中加入纯化的牛LPL来补充LPL导致野生型和LPL敲低细胞中ABCA 1介导的胆固醇流出的下调。这些发现表明巨噬细胞LPL水平与ABCA 1胆固醇转运活性之间存在负相关性。
The ATP-binding cassette transporter A1 (ABCA1) mediates the efflux of excess cholesterol from foam cells to lipid-poor apolipoprotein A-I, in a process called reverse cholesterol transport. Lipoprotein lipase (LPL) is a lipolytic enzyme expressed by macrophages within atherosclerotic lesions. Lentivirus-mediated RNA interference was used to genetically knock-down (KD) the expression of LPL in THP-1 macrophages. Silencing of the LPL gene was confirmed by end-point PCR, real time PCR, and protein analysis. Suppression of LPL expression correlated with a 1.6-fold up-regulation of ABCA1 mRNA levels, and resulted in a 4.5-fold increase in ABCA1-dependent cholesterol efflux. Replenishing LPL by addition of purified bovine LPL to the cell culture media resulted in down-regulation of ABCA1-mediated cholesterol efflux in both wild-type and LPL knockdown cells. These finding suggests an inverse correlation between macrophage LPL levels and ABCA1 cholesterol transport activity.
登录
查看更多内容
影响因子：
5.3
作者：
Ichikawa, T;Liang, JY;Fan, JL
通讯作者：
Fan, JL
影响因子：
5.3
作者：
Uehara, Yoshinari;Miura, Shin-ichiro;Saku, Keijiro
通讯作者：
Saku, Keijiro
影响因子：
8.7
作者：
Van Eck, M;Zimmermann, R;Van Berkel, TJC
通讯作者：
Van Berkel, TJC
影响因子：
4.8
作者：
Babaev, VR;Patel, MB;Linton, MF
通讯作者：
Linton, MF
影响因子：
5.6
作者：
Lee, Jiyoung;Park, Youngki;Koo, Sung I.
通讯作者：
Koo, Sung I.