4-Hydroxynonenal inhibits cell proliferation and alters differentiation pathways in human fetal liver hematopoietic stem cells.
4-Hydroxynonenal inhibits cell proliferation and alters differentiation pathways in human fetal liver hematopoietic stem cells.
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4-Hydroxynonenal 抑制细胞增殖并改变人胎肝造血干细胞的分化途径。
DOI：
10.1016/j.bcp.2004.09.001
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发表时间：
2005
影响因子：
5.8
通讯作者：
Gallagher,EvanP
中科院分区：
文献类型：
--
作者：
Moneypenny,CraigG;Gallagher,EvanP
在胎儿发育期间，肝脏作为主要造血器官，其中造血干细胞（HSC）构成大比例的肝细胞群。由于HSC能够启动长期造血，这些细胞的损伤可能与血源性疾病的病因学有关。在目前的研究中，我们研究了4-羟基壬烯醛（4-HNE），一种可在子宫内产生的致突变α，β-不饱和醛，对HSC增殖，分化，活力和凋亡的影响。HSC暴露于低至1 nM的急性单剂量4-HNE可抑制HSC增殖。由于4-HNE从培养基中迅速消失，因此还采用了多次给药方案，以接近与生理氧化应激相关的短期稳态4-HNE浓度。4-低至1μM的羟基壬烯醛稳态浓度改变了HSC分化途径，但不影响凋亡或引起细胞死亡。相比之下，暴露于稳态5μM 4-HNE引起存活力的丧失，并增加总HSC群体的凋亡率。总的来说，我们的数据表明，与低水平的氧化应激相关的细胞水平的4-HNE导致增殖和活力的损失，并改变人胎儿HSC的分化途径。
During fetal development, the liver serves as the primary hematopoietic organ in which hematopoietic stem cells (HSC) comprise a large proportion of hepatic cell populations. Because HSC are capable of initiating long-term hematopoiesis, injury to these cells may have ramifications with regard to the etiology of blood-borne diseases. In the current study, we examined the effects of 4-hydroxynonenal (4-HNE), a mutagenic α,β-unsaturated aldehyde that can be produced in utero, on HSC proliferation, differentiation, viability and apoptosis. Exposure of HSC to acute single doses of 4-HNE as low as 1nM inhibited HSC proliferation. Because 4-HNE rapidly disappears from culture media, a multiple dosing régime was also employed to approximate short-term steady state 4-HNE concentrations relevant to physiological oxidative stress. 4-Hydroxynonenal steady state concentrations as low as 1μM altered HSC differentiation pathways, but did not affect apoptosis or cause cell death. In contrast, exposure to steady state 5μM 4-HNE elicited a loss in viability, and increased the rate of apoptosis in total HSC populations. Collectively, our data indicate that cellular levels of 4-HNE associated with a low level of oxidative stress cause a loss of proliferation and viability and alter differentiation pathways in human fetal HSC.
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影响因子：
10.6
作者：
Y. Awasthi;Rajendra K. Sharma;Ji-Zhong Cheng;Yusong Yang;Abha Sharma;S. Singhal;S. Awasthi
通讯作者：
Y. Awasthi;Rajendra K. Sharma;Ji-Zhong Cheng;Yusong Yang;Abha Sharma;S. Singhal;S. Awasthi
氢过氧化异丙苯和 4-羟基壬烯醛对 DNA 和蛋白质合成的可逆抑制
影响因子：
5.3
作者：
M. Poot;A. Verkerk;J. Koster;H. Esterbauer;J. F. Jongkind
通讯作者：
J. F. Jongkind
C57/BL6 小鼠短期高浓度饮食乙醇导致淋巴细胞亚群和巨噬细胞功能发生变化。
DOI：
--
发表时间：
1988
期刊：
Life Science
影响因子：
--
作者：
Ronald R. Watson;Rao H. Prabhala;Edward R. Abril;Tom L. Smith
通讯作者：
Tom L. Smith
人类祖细胞液体培养过程中 CD34+ 细胞的扩增和谱系标记物的表达
影响因子：
5.2
作者：
M. Warren;W. L. Rose;L. Beall;J. Cone
通讯作者：
J. Cone
影响因子：
3.7
作者：
BARRERA, G;DIMAURO, C;DIANZANI, MU
通讯作者：
DIANZANI, MU