Functional characterization of the upstream components of the Hog1-like kinase cascade in hyperosmotic and carbon sensing in Trichoderma reesei.
Functional characterization of the upstream components of the Hog1-like kinase cascade in hyperosmotic and carbon sensing in Trichoderma reesei.
复制标题
里氏木霉高渗和碳传感中 Hog1 样激酶级联上游成分的功能表征
DOI：
10.1186/s13068-018-1098-8
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发表时间：
2018
影响因子：
6.3
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Wang Z;An N;Xu W;Zhang W;Meng X;Chen G;Liu W
研究背景里氏木霉（Trichoderma reesei）是纤维素酶的重要生产菌，具有较高的蛋白分泌能力。然而，在纤维素诱导过程中的外部信号感应和细胞内信号转导仍不清楚。丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）途径及其上游传感和信号传导元件是所有真核生物中最普遍的信号传导途径之一，参与包括应激和营养传感在内的多种生理过程。特别地，Hog 1型MAPK Tmk 3已被报道参与T.结果确定了Tmk 3信号通路的两个上游调控分支Sho 1分支和Sln 1分支在T. reesei。缺失Sho 1分支的Trste 20或阻遏Sln 1分支的Trypd 1均降低了对高盐胁迫的抗性，而TrSho 1则表现出与TrSte 20相反的作用，并且所鉴定的TrSln 1似乎在渗透调节中起着相反的作用。Sho 1和Sln 1分支还参与细胞壁完整性的维持和其他应激反应（即氧化和热应激）。值得注意的是，Sho 1分支的TrSho 1和TrSte 20以及Sln 1分支的TrYpd 1显示出差异地参与T. reesei。Trypd 1的抑制几乎不影响纤维素酶的诱导，而Trypd 1的过表达导致纤维素酶的产量减少。结论Sho 1分支的TrSho 1和TrSte 20以及Sln 1分支的TrYpd 1均参与了高渗调节和细胞壁完整性维持等一般性的胁迫反应。此外，我们的研究表明，Sho 1和Sln 1 β传感途径在T. reesei。Sho 1分支正调控纤维素酶的产生和纤维素酶基因的转录，而Sln 1分支的TrYpd 1负调控纤维素酶的产生，支持了植物传感和营养传感的交叉。
BackgroundTrichoderma reesei holds a high capacity for protein secretion and represents the most important cellulase producer in industry. However, the external signal sensing and intracellular signal transduction during cellulose induction remain unclear. As one of the most pervasive signal transduction pathways in all eukaryotic species, the mitogen-activated protein kinase (MAPK) pathway and its upstream sensing and signaling components are involved in various physiological processes including stress and nutrient sensing. Particularly, the Hog1-type MAPK Tmk3 has been reported to be involved in the cellulase production in T. reesei.ResultsHere we established the physiological role of two upstream regulatory branches, the Sho1 branch and the Sln1 branch, of the Hog1-type Tmk3 pathway in T. reesei. Deletion of Trste20 of the Sho1 branch or repression of Trypd1 of the Sln1 branch reduced the resistance to high salt stress, whereas TrSho1 showed an opposing effect to that of TrSte20 and the identified TrSln1 seemed to be dispensable in the osmotic regulation. The Sho1 and Sln1 branches also participated in the cell wall integrity maintenance and other stress responses (i.e. oxidative and thermo stresses). Notably, TrSho1 and TrSte20 of the Sho1 branch and TrYpd1 of the Sln1 branch were shown to be differentially involved in the cellulase production of T. reesei. Repression of Trypd1 hardly affected cellulase induction, whereas overexpression of Trypd1 resulted in the reduced production of cellulases. Contrary to the case of Trypd1, repression of Trsho1 or deletion of Trste20 significantly reduced the transcription of cellulase genes.ConclusionsTrSho1 and TrSte20 of the Sho1 branch and TrYpd1 of the Sln1 branch are all involved in general stress responses including hyperosmotic regulation and cell wall integrity maintenance. Moreover, our study revealed that the Sho1 and Sln1 osmosensing pathways are differentially involved in the regulation of cellulase production in T. reesei. The Sho1 branch positively regulated the production of cellulases and the transcription of cellulase genes while TrYpd1 of the Sln1 branch negatively controlled the cellulase production, supporting the crosstalks of osmosensing and nutrient sensing.
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