Insight into the interaction between the RNA helicase CGH-1 and EDC-3 and its implications.
Insight into the interaction between the RNA helicase CGH-1 and EDC-3 and its implications.
复制标题
深入了解 RNA 解旋酶 CGH-1 和 EDC-3 之间的相互作用及其影响
DOI：
10.1038/s41598-021-99919-0
复制
发表时间：
2021-10-13
影响因子：
4.6
通讯作者：
Hong J
中科院分区：
文献类型：
--
作者：
Zhang Y;Wang K;Yang K;Shi Y;Hong J
以前的研究表明，P体组分CGH-1和EDC-3可能在调节秀丽线虫寿命的过程中发挥关键作用。Elegans)分别与EDC3(酵母中的Edc3p)形成络合物，这对翻译抑制和mRNA衰变具有重要意义。然而，目前尚不清楚EDC-3 Inc.如何识别CGH-1。优雅女装。在这里，我们提供了关于CGH-1和EDC-3之间相互作用的结构和生化方面的见解。结合同源模建、突变和ITC分析，我们发现了CGH-1RecA2结构域和EDC-3FDF-Fek之间的界面。此外，GST-Pull和共定位实验证实了CGH-1和EDC-3在体内外的相互作用。我们还用ITC方法分析了CGH-1RecA2上的PATR-1结合界面。此外，我们还揭示了EDC-3与CAR-1或PATR-1结合模式的异同。综上所述，这些发现为EDC-3FDF-FEK基序对DEAD-box蛋白CGH-1的识别提供了见解，提示了重要的功能意义。
Previous studies indicated that the P-body components, CGH-1 and EDC-3 may play a crucial role in the regulation of lifespan inCaenorhabditis elegans.Homo sapiensDDX6 orSaccharomyces cerevisiaeDhh1p (CGH-1 inC. elegans) could form complexes with EDC3 (Edc3p in yeast), respectively, which is significant for translation inhibition and mRNA decay. However, it is currently unclear how CGH-1 can be recognized by EDC-3 inC. elegans. Here, we provided structural and biochemical insights into the interaction between CGH-1 and EDC-3. Combined with homology modeling, mutation, and ITC assays, we uncovered an interface between CGH-1 RecA2 domain and EDC-3 FDF-FEK. Additionally, GST-pulldown and co-localization experiments confirmed the interaction between CGH-1 and EDC-3 in vitro and in vivo. We also analyzed PATR-1-binding interface on CGH-1 RecA2 by ITC assays. Moreover, we unveiled the similarity and differences of the binding mode between EDC-3 and CAR-1 or PATR-1. Taken together, these findings provide insights into the recognition of DEAD-box protein CGH-1 by EDC-3 FDF-FEK motif, suggesting important functional implications.
登录
查看更多内容
影响因子：
5.3
作者：
Tritschler, Felix;Eulalio, Ana;Truffault, Vincent
通讯作者：
Truffault, Vincent
DOI：
10.1073/pnas.1509499112
发表时间：
2015-12-29
影响因子：
11.1
作者：
Alessi, Amelia F.;Khivansara, Vishal;Kim, John K.
通讯作者：
Kim, John K.
影响因子：
3.9
作者：
Kulkarni, Meeta;Ozgur, Sevim;Stoecklin, Georg
通讯作者：
Stoecklin, Georg
影响因子：
5.3
作者：
Tritschler, Felix;Eulalio, Ana;Weichenrieder, Oliver
通讯作者：
Weichenrieder, Oliver
影响因子：
5.3
作者：
Ling, Sharon H. M.;Decker, Carolyn J.;Song, Haiwei
通讯作者：
Song, Haiwei