The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice.
The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice.
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进化上保守的基因：Tex37、Ccdc73、Prss55 和 Nxt2 对于小鼠的生育能力来说是可有可无的
DOI：
10.1038/s41598-018-23176-x
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发表时间：
2018-03-21
影响因子：
4.6
通讯作者：
Shi Q
中科院分区：
文献类型：
--
作者：
Khan M;Jabeen N;Khan T;Hussain HMJ;Ali A;Khan R;Jiang L;Li T;Tao Q;Zhang X;Yin H;Yu C;Jiang X;Shi Q
有超过2300个基因主要在小鼠睾丸中表达。数百个这样的基因在小鼠精子发生中的作用已经被研究，但仍然有许多基因的功能尚不清楚。小鼠基因敲除(KO)策略被广泛应用于基因功能的体内研究。本研究旨在探索在真兽类进化中保守的4个基因：Tex37、Ccdc73、Prss 55和NXT2的功能。我们发现，除了NXT2外，这些基因在小鼠中都有睾丸丰富的表达模式。我们通过CRISPR/Cas9分别敲除了这些基因，发现所有KO小鼠都具有正常的生育力，在睾丸/体重比、附睾精子计数以及睾丸和附睾组织学上与野生型小鼠没有明显差异。虽然这些基因在包括人类和小鼠在内的真兽类动物中进化上是保守的，但它们并不是实验室条件下小鼠精子发生、睾丸发育和雄性生育所必需的个体。我们对这些肥沃的KO数据的报告可以避免重复和重复的努力，这将有助于优先关注对男性生殖不可或缺的基因。
There are more than 2300 genes that are predominantly expressed in mouse testes. The role of hundreds of these genes has been studied in mouse spermatogenesis but still there are many genes whose function is unknown. Gene knockout (KO) strategy in mice is widely used for in vivo study of gene function. The present study was designed to explore the function of the four genes: Tex37, Ccdc73, Prss55 and Nxt2, which were evolutionarily conserved in eutherians. We found that these genes had a testis-enriched expression pattern in mice except Nxt2. We knocked out these genes by CRISPR/Cas9 individually and found that all the KO mice had normal fertility with no detectable difference in testis/body weight ratios, epididymal sperm counts, as well as testicular and epididymal histology from wild type mice. Although these genes are evolutionarily conserved in eutherians including human and mouse, they are not individually essential for spermatogenesis, testis development and male fertility in mice in laboratory conditions. Our report of these fertile KO data could avoid the repetition and duplication of efforts which will help in prioritizing efforts to focus on genes that are indispensable for male reproduction.
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血睾屏障和精子发生：转基因小鼠的教训
影响因子：
2.9
作者：
Jiang XH;Bukhari I;Zheng W;Yin S;Wang Z;Cooke HJ;Shi QH
通讯作者：
Shi QH
影响因子：
11.1
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通讯作者：
Arnoult C
影响因子：
4.5
作者：
Ollinger R;Childs AJ;Burgess HM;Speed RM;Lundegaard PR;Reynolds N;Gray NK;Cooke HJ;Adams IR
通讯作者：
Adams IR
DOI：
10.1073/pnas.1635054100
发表时间：
2003-10-14
影响因子：
11.1
作者：
Schultz, N;Hamra, FK;Garbers, DL
通讯作者：
Garbers, DL
影响因子：
4
作者：
Ayhan, Ozgecan;Balkan, Mahmut;Tolun, Aslihan
通讯作者：
Tolun, Aslihan