Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes.
Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes.
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DOI：
10.1186/s12943-017-0730-8
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发表时间：
2017-10-13
期刊：
影响因子：
37.3
通讯作者：
Scala G
中科院分区：
文献类型：
--
作者：
Iaccino E;Mimmi S;Dattilo V;Marino F;Candeloro P;Di Loria A;Marimpietri D;Pisano A;Albano F;Vecchio E;Ceglia S;Golino G;Lupia A;Fiume G;Quinto I;Scala G
肿瘤源性外泌体（Tumor-derived exosomes，TDEs）在肿瘤的形成和发展中起着关键作用，是个体化医学中肿瘤诊断的新兴生物标志物。迄今为止，缺乏用于外泌体分离和表征的有效技术平台。多发性骨髓瘤（MM）是一种无法治愈的B细胞恶性肿瘤，由于迅速发展的耐药性。MM释放的外泌体表达肿瘤B细胞的免疫球蛋白B细胞受体（Ig-BCR），其可以被特发性结合肽（Id-肽）靶向。在这项研究中，我们分析了在小鼠5 T33 MM多发性骨髓瘤模型中MM释放的外泌体作为肿瘤生长生物标志物的产生。为此，我们通过使用由5 T33 MM细胞表达的Ig-BCR作为诱饵筛选噬菌体展示文库来选择Id-肽。通过FACS，FITC缀合的Id-肽检测到5 T33 MM移植小鼠血清中MM释放的外来体，与血清副蛋白相比，其水平与较早时间点的肿瘤进展相关。这些结果表明，MM释放的外泌体的基于Id肽的识别可能代表用于疾病进展的临床评价的非常敏感的诊断方法。本文的在线版本（10.1186/s12943-017-0730-8）包含补充材料，可供授权用户使用。
Tumor-derived exosomes (TDEs) play a pivotal role in tumor establishment and progression, and are emerging biomarkers for tumor diagnosis in personalized medicine. To date, there is a lack of efficient technology platforms for exosome isolation and characterization. Multiple myeloma (MM) is an incurable B-cell malignancy due to the rapid development of drug-resistance. MM-released exosomes express the immunoglobulin B-cell receptor (Ig-BCR) of the tumor B-cells, which can be targeted by Idiotype-binding peptides (Id-peptides). In this study, we analyzed the production of MM-released exosomes in the murine 5T33MM multiple myeloma model as biomarkers of tumor growth. To this end, we selected Id-peptides by screening a phage display library using as bait the Ig-BCR expressed by 5T33MM cells. By FACS, the FITC-conjugated Id-peptides detected the MM-released exosomes in the serum of 5T33MM-engrafted mice, levels of which are correlated with tumor progression at an earlier time point compared to serum paraprotein. These results indicate that Id-peptide-based recognition of MM-released exosomes may represent a very sensitive diagnostic approach for clinical evaluation of disease progression. The online version of this article (10.1186/s12943-017-0730-8) contains supplementary material, which is available to authorized users.
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影响因子：
--
作者：
Arita T;Ichikawa D;Konishi H;Komatsu S;Shiozaki A;Ogino S;Fujita Y;Hiramoto H;Hamada J;Shoda K;Kosuga T;Fujiwara H;Okamoto K;Otsuji E
通讯作者：
Otsuji E
DOI：
10.1083/jcb.101.3.942
发表时间：
1985-09
期刊：
The Journal of cell biology
影响因子：
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通讯作者：
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影响因子：
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通讯作者：
Whiteside TL
影响因子：
30.3
作者：
Muratori, Claudia;Cavallin, Lucas E.;Baur, Andreas S.
通讯作者：
Baur, Andreas S.
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影响因子：
30.5
作者：
Li, Jianhua;Liu, Kuancheng;Yuan, Zhenghong
通讯作者：
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